As urbanization increases and natural access decreases, stress levels among individuals are rising. Previous literature suggests that nature exposure, whether real or simulated, can improve both physiological well-being. This study explored whether short-term exposure to immersive virtual reality environments, specifically nature-based versus urban landscapes, could influence stress levels. A within-subject design was used in which 20 participants experienced both nature and an urban VR condition. Stress was measured using self-reported data from the State-Trait Anxiety Inventory and physiological data from heart rate variability. Participants reported significantly lower subjective stress following the nature VR condition compared to both the baseline and urban VR conditions. However, no significant differences in heart rate variability were observed between the nature and baseline conditions, nor between the nature and urban VR conditions.