Differences in immunity between males and females is one of the factors that contribute to sex bias in diseases. Females of reproductive age are more likely to experience worse outcomes from pandemic influenza A virus (IAV) infection compared to males. One of the possible explanations is the effect of high estrogen levels during that life period. It is known that estrogen affects immune cells, but the mechanism on T and B cells is thought to be indirect. The aim of this project was to investigate if this effect can be attributed to estrogen signaling in lymph node stromal cells. For this purpose, female control mice and mice with conditional knockout (cKO) of estrogen receptor alpha in fibroblastic reticular cells (FRCs), a subset of lymph node stromal cells, were infected with IAV or administered mock treatment. Mice were weighed daily to monitor disease progression and were euthanized on day 6 post-infection. The mediastinal lymph node was dissected, digested and analyzed by flow cytometry focusing on FRCs, T cells and B cells. The left lung lobe was collected, histologically processed and microscopically analyzed. Infected cKO mice showed significant differences for most of the examined parameters and appeared to have mounted a stronger immune response, however, only B cell counts showed significant difference between the genotypes. To summarize, due to a small sample size and lack of statistical significance, a definitive conclusion about the effects of estrogen on IAV-induced inflammation cannot be made.