Högskolan i Skövde

his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Exploring SOX11-SAMHD1 interaction in lymphoid malignancies: Computational approaches to binding interface prediction, transcriptomics, and synthetic lethality targets
University of Skövde, School of Bioscience.
2025 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Mantle cell lymphoma (MCL) and Burkitt lymphoma (BL) are aggressive lymphoid malignancies with distinct molecular features and variable treatment outcomes. The transcription factor, SOX11 is atypically expressed in the majority of MCL cases and a subset of BL, and has emerged as a key regulator of gene expression and tumor biology. Recent findings identified SAMHD1, a dNTPase involved in nucleotide metabolism and DNA repair, as a novel interacting partner of SOX11. This thesis explores the structural and functional dynamics of the SOX11–SAMHD1 interaction using a multidisciplinary approach integrating transcriptomics, proteomics, computational modeling, and molecular validation. RNA-seq analysis of SOX11-expressing BLcell lines revealed over 5,600 differentially expressed genes, with significant enrichment for DNA damage response and immune regulation pathways. Proteomic profiling of MCL cell lines identified 51 high-confidence SOX11-binding proteins involved in RNA splicing and nuclear structure, with SAMHD1 among the top candidates. Molecular docking using HADDOCK predicted a stable electrostatically-driven interaction interface between SOX11 and SAMHD1, which was further characterized by PDBsum analysis. Native gel electrophoresis demonstrated that SOX11 impairs SAMHD1 tetramerization in MCL cells, but not in BL cells, corroborating the context-specificity of this interaction. Proximity ligation assays confirmed nuclear co-localization of SOX11 and SAMHD1 only in MCL models, but not in BL. These findings suggest broader roles for SOX11 in RNA processing and genome stability. This work offers novel insights into the molecular pathology of lymphoid malignancies and proposes the SOX11–SAMHD1 axis as a targetable vulnerability in precision oncology.

Place, publisher, year, edition, pages
2025. , p. 36
National Category
Medical Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:his:diva-25233OAI: oai:DiVA.org:his-25233DiVA, id: diva2:1971623
External cooperation
Karolinska Institute
Subject / course
Systems Biology
Educational program
Molecular Biotechnology - Master's Programme, 120 ECTS
Supervisors
Examiners
Available from: 2025-06-17 Created: 2025-06-17 Last updated: 2025-09-29Bibliographically approved

Open Access in DiVA

fulltext(2840 kB)121 downloads
File information
File name FULLTEXT01.pdfFile size 2840 kBChecksum SHA-512
3408ac45e43d81e70702b31d22dc4f434de8d3809a52286b9c6917d3cb134e0a57f2537600d54ddd77f76bec62e34966f1ba5c3cbed0f87ba0e2f55d653eb5a0
Type fulltextMimetype application/pdf

By organisation
School of Bioscience
Medical Bioinformatics and Systems Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 121 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 204 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf