Adiponectin, a key regulatory hormone, modulates metabolic processes and significantly affects pregnancy outcomes. This thesis investigates the impact of elevated maternal adiponectin levels on the phosphoproteome of fetal liver and placental tissues, aiming to show the molecular mechanisms underlying adiponectin's influence on pregnancy. This study aims to analyze the changes in the phosphoproteome of fetal liver and placental tissues in response to adiponectin overexpression, identifying pathways and key kinases involved in regulating these changes. Phosphoproteomic analysis was performed on samples from pregnant wild-type (WT) and transgenic (TG) dams overexpressing adiponectin. Differential expression analysis identified significant phosphosites, which were subjected to Gene Ontology (GO) enrichment and kinase-substrate relationship analysis using the PhosR package by R. The signalome was performed to map the interactions and regulatory roles of key kinases. GO enrichment analysis revealed significant biological processes influenced by adiponectin in both fetal liver and placental tissues. In the fetus, processes such as chromatin remodeling were prominently enriched. In the placenta, RNA-related processes including mRNA processing and regulation of RNA stability were significantly enriched. The kinase-substrate relationship analysis highlighted the pivotal roles of CMGC kinases, particularly MAPK1 and 3, in both fetal liver and placental tissues. In the placenta, AGC kinases and MTOR were significantly regulated, indicating their roles in regulating metabolism, cell growth, and apoptosis. Signalome analysis revealed networks of kinase interactions, with key modules identified in both fetal and placental tissues that are involved in critical regulatory processes. Overexpressed maternal adiponectin significantly influences the phosphoproteome of fetal liver and placental tissues, affecting key biological processes and regulatory pathways. The findings illustrate the crucial roles of specific kinases and pathways in these effects, providing insights into the molecular mechanisms underlying adiponectin's influence on placenta nutrient transport and fetal growth. Future research should focus on validating these findings experimentally and exploring the therapeutic potential of adiponectin modulation in obesity-related pregnancy complications.