Sexually transmitted infections (STIs) represent a significant global health concern, with Human Papillomavirus (HPV) being one of the most prevalent pathogens and contributing significantly to the development of cervical cancer. Cervical cancer ranks as the second most common cancer in women globally. HPV vaccination is effective; however, challenges exist in adopting HPV vaccination programmes in most developing countries. Neutrophils, key players in inflammation, influence tumour progression, with elevated neutrophil-to-lymphocyte ratios linked to advanced cancers and poor outcomes. The role of neutrophils in the development of HPV infection and cervical cancer has also been demonstrated. This study aimed to investigate neutrophil activation by different squamous cell carcinoma cell lines with a specific focus on the impact of HPV. The methodology involved cultivating the cervical cancer cell lines C33A, Caski, HeLa, and SiHa. Neutrophils were then isolated from buffy coats and exposed to lysates and supernatants from the cell lines, followed by analysis for apoptosis and necrosis. Furthermore, the surface markers, CD11b and CD62l, on neutrophils were stained and examined using flow cytometry. The findings of this study revealed that HeLa and Caski cell supernatants induced a decrease in neutrophil apoptosis, suggesting an anti-apoptotic effect. HeLa supernatant specifically activated neutrophils, as indicated by alterations in the surface markers CD11b and CD62l. Further investigation is warranted to delineate these mechanisms and their relevance for the immune response to cervical cancer.