Virtual animal violence is a subject that has garnered relatively little attention in the greater discussion of general violence in video games. The subject of virtual animal violence offers avenues for examining moral ambiguities of play from a perhaps novel perspective. This thesis specifically examines the experiences of vegetarians when committing acts of virtual violence towards animals. Vegetarians as a group have historically been overlooked in terms of Games User Research (GUR), as is demonstrated by the small body of research pertaining to them. To study this group, in-depth semi-structured interviews were conducted with six participants. Through inductive content analysis this thesis aimed to deepen our understanding of moral ambiguity and broaden the discussion of video game violence. The analysis showed that vegetarian players experience varied degrees of emotional distress and moral dissonance when harming virtual animals. Themes and concepts were identified that encapsulate what vegetarian players experience with regards to morals and emotion. This thesis concludes that a negative emotional effect on players when harming virtual animals is present. However, it is not significant enough for the player’s overall gaming experience to be seen as negative. The analysis also revealed potential research avenues for future studies.