Neoehrlichia mikurensis is a bacterium transmitted to humans by tick bites, usually from Ixodes ricinus species, causing an infection known as neoehrlichiosis. The importance of B cells in host defence against this infection has previously been demonstrated in patients undergoing anti-B cell therapy for lymphoma. As a causal relationship between N. mikurensis and malignant B-cell lymphomas has not yet been established, the identification of a genetic signature caused by the infection would be a step forward in the development of therapeutics. Findings have been published on the heavy chain repertoire of the major B-cell clonotypes of N. mikurensis-positive lymphoma patients, and it is essential to gain a deeper understanding of the broader heavy chain repertoire. That can be achieved by analysing other aspects such as VDJ gene frequency, somatic hypermutations in complementarity determining regions (CDRs) and framework regions (FWRs) of the variable domain, and the physicochemical properties of the H-CDR3 repertoire, the latter being the most hypervariable part of the heavy chain and a major determinant of its specificity. Specifically, eight infected lymphoma patients were analysed for these variables. In short, the analysis of the results separates the data into at least two distinct groups of patients studied. It was also possible to group the patients by integrating the data into heatmap representations that clustered the patients in an unsupervised manner based on the use of IGHV, IGHD and IGHJ gene frequencies and the physicochemical properties of H-CDR3. Interestingly, this correlated closely with the lymphoma classifications of the patients infected with N. mikurensis. Although the sample size is not very large, and work is currently being to recruit more patients and controls, these results are promising as not only may there be an underlying signature related to the infection, but they may also help to separate the heavy chain repertoire data into groups, which in turn may help to diagnose lymphoma patients more accurately and perhaps more quickly and find the right treatment from the outset.