Apis mellifera are a key contributor to many different and important ecosystems around the world which is something it has in common with other pollinator species. In a very unfortunate turn of events, these pollinator species show a decline in numbers. The decline of which can be partially attributed to human interference through actions that creates habitat loss and pesticide exposure. With the Apis mellifera being an integral part of numerous ecosystems and important for agriculture, their decline is concerning. Research into the Apis mellifera has shown that their gut microbiota has a large impact on their health. With the health of the Apis mellifera being more important than ever before, research into their gut microbiota is of key importance. The aim of this study was to investigate the gut microbiota in different individuals from the same hive and compare them. Investigation of the gut microbiota was achieved through 16S rRNA sequencing of the mid and hindgut with the use of Nanopore sequencing. Results of the sequencing showed high similarities with regards to the bacterial species found, however there were varying proportions of the species in the individuals. The observed similarities were investigated though PCA which confirmed this observation. In conclusion, individuals from the same hive show large similarities in bacterial species with proportions differing being a natural part of the Apis mellifera lifecycle.