Ulcerative colitis is characterised by chronic inflammation in the colon and rectum. The incidence of ulcerative colitis has been steadily increasing among young adults globally. Symptoms of the disease include abdominal pain, diarrhea, and recurrent rectal bleeding, with periods of remission interspersed with active disease states. The pathogenesis of ulcerative colitis involves a complex interplay of genetic predisposition and environmental factors. The key contributors to the disease development are epithelial barrier dysfunction, dysregulated immune response, and microbial dysbiosis. This is a pilot study that investigated the effects of fecal supernatants from patients with ulcerative colitis on memory T-cell activation using covid antigen obtained from a healthy blood donor as a model recall antigen. Caco-2 cell monolayers were differentiated into enterocytes and exposed to fecal supernatants. Peripheral blood mononuclear cells from a healthy donor were cultured with conditioned media obtained from Caco-2 cells or with fecal supernatants directly. Flow cytometry was performed to evaluate the difference in memory T-cell activation. The analysis of flow cytometry results revealed reduced T-cell activation upon exposure to fecal supernatants from active ulcerative colitis patients compared to healthy subjects, both directly and via conditioned media from Caco-2 cells. The study also provides insights into the optimal fecal supernatant dilutions to be used for Caco-2 cell stimulation. The reduced memory T-cell activation reflects a dysregulated immune response associated with ulcerative colitis.