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Identification of Gene Modules in Type 2 Diabetes by Integrating Transcriptomics into Gene Interaction Network Analysis
University of Skövde, School of Bioscience.
2025 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Type 2 Diabetes (T2D) is a complex metabolic disorder characterized by coordinated dysregulation of multiple genes and biological pathways. Although transcriptomic technologie senable large-scale analysis of gene expression changes in T2D, many studies rely primarily on differential gene expression analysis, which often fails to capture gene–gene interactions and systems-level regulatory mechanisms. To address this limitation, this study applied a transcriptomics-based gene interaction network approach to investigate the molecular architecture of T2D. Publicly available RNA-sequencing data from T2D patients and healthy controls were analyzed using established bioinformatics pipelines. After quality control and normalization, differential expression analysis was performed, followed by the construction of gene co-expression networks and identify functionally related gene modules. Disease associated modules were further examined to detect highly connected hub genes based on network topology and connectivity measures. Functional enrichment analysis of hub genes using Gene Set Enrichment Analysis (GSEA) was not feasible, as the available data did not meet the input requirements of the method; therefore, pathway-level enrichment was performed using GSEA on a ranked gene list derived from differential expression statistics. Network robustness was assessed through connectivity strength and network density analyses to ensure reliability. The analysis identified distinct gene modules and central hub genes associated with metabolic regulation, mitochondrial function, and cellular homeostasis relevant to T2D. Overall, this study demonstrated that network-based transcriptomic analysis provides deeper biological insights than traditional gene-centric approaches and offers a systems-level framework for understanding the molecular mechanisms underlying T2D.

Place, publisher, year, edition, pages
2025. , p. 35
National Category
Bioinformatics and Computational Biology
Identifiers
URN: urn:nbn:se:his:diva-26237OAI: oai:DiVA.org:his-26237DiVA, id: diva2:2050171
Subject / course
Bioinformatics
Educational program
Bioinformatics - Master’s Programme
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Available from: 2026-04-01 Created: 2026-04-01 Last updated: 2026-04-01Bibliographically approved

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1920212223242522 of 36
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf