As longevity can be influenced by both genetics and external factors, a healthy lifestyle, including an appropriate diet, could help extend one’s lifespan. Given that diets such as the Mediterranean diet have already been associated with improved overall health, investigating their effects on telomere maintenance, oxidative stress and inflammation is particularly relevant. This study aimed at determining the effect of diets such as the Mediterranean diet on longevity by analyzing blood markers and genes linked to longevity. To achieve this, C-reactive protein, homocysteine, citrulline, glycine, methionine, glucose levels and genes such as TERT, CISD2 and FOXO3 were investigated. For four weeks, a group of five students followed a diet according to the set macronutrient percentages: 40% carbohydrates, 35% proteins and 25% fats. Moreover, a control group including five additional students was recruited. The effects of diets were studied by sending blood samples to external labs for analyses of blood markers and amino acids, analyses which were done by examining basal physiological parameters and by measuring gene expression through quantitative PCR. The results did not show any significant changes for all the investigated parameters in either the intervention or control group after the diet. Therefore, no conclusive statements could be made, highlighting the need for further research with a longer diet intervention period and a larger sample size.