Sepsis is one of the main causes of death all around the world and could be caused by different types of infections, like viral, bacterial or fungal. Time is of essence when a patient goes septic, usually leaving a few hours only to deliver appropriate treatment. As antimicrobial resistance is becoming more common in recent years, the right treatment being delivered on time is important. Current culture-based diagnosis methods could take 24-72 hours for results and often that might be too late. This is why usually a preliminary treatment is delivered until results are back and this could be not effective if antimicrobial resistance is present in the ongoing infection. That is one of the reasons that researchers have chosen to explore and study other methods like metagenomic Next-Generation Sequencing where DNA is extracted directly from bodily fluid or tissue sample and sequenced and then analyzed. In this study urine from a healthy volunteer was collected and then spiked with different types of spike-ins. DNA was then extracted using two kits, and the DNA extracted was sequenced using the nanopore sequencing technique. The data was then analyzed using EPI2ME software. The results showed the potential this technique can have in clinical applications. In higher spike-in concentrations all expected bacteria species were identified, but not all genes expected that could confer antimicrobial resistance. The results of the experiment showed the significance of the DNA extraction step as the shearing of DNA during extraction lead to shorter and lower quality sequencing reads. This approach opens new doors to healthier communities, equality and sustainability. However, further research and exploration are needed to improve this approach and make it more reliable for clinical applications.