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  • 1051.
    Sundqvist, Robert
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Khan, Fahima
    Högskolan i Skövde, Institutionen för vård och natur.
    ATT VARA NÄRSTÅENDE TILL PERSONER MED CANCER: En litteraturöversikt2009Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

     

    Var tredje person i Sverige kan under sin livstid drabbas av en cancersjukdom. När en familjmedlem får diagnosen cancer påverkar detta oftast livet för dem som är närstående. Forskning visar att de närstående upplever olika förändringar i livet. Det uppstår oftast känslor som ångest, hjälplöshet och maktlöshet. Syftet med denna litteraturöversikt var att beskriva hur närstående till personer med cancer upplever sin förändrade livssituation. Informationskällor använda i analysen är 13 artiklar som beskriver närståendes upplevelser. Resultatet presenteras i fyra olika huvudteman med tillhörande underteman. Dessa fyra teman är Förändrad livssituation, Maktlöshet och hjälplöshet, Stöd är betydelsefullt och Behov av information. I resultatet framgår att relationer mellan familjmedlemmar antingen fördjupas eller försämras. Behovet av information och stöd visar sig vara stort hos den närstående. Det är av betydelse att resultatet används i vården så att sjuksköterskor samt annan vårdpersonal blir uppmärksamma på närståendes upplevelse. Dessa kunskaper kan hjälpa sjuksköterskor och annan vårdpersonal att erbjuda bättre stöd till dessa närstående.

    Fulltekst (pdf)
    FULLTEXT01
  • 1052.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Habitat requirements and distribution of wood-and bark inhabiting fungi on oak2006Inngår i: The oak: history, ecology, management and planning : proceedings from a conference in Linköping, Stockholm: Naturvårdsverket , 2006, s. 24-25Konferansepaper (Annet vitenskapelig)
  • 1053.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek - 11: Igelkottaggsvamp (Hericium erinaceum)2004Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, Vol. 19, nr 1, s. 32-36Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1054.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek - 16. Platticka2009Inngår i: Ekbladet, ISSN 0283-4839, nr 24, s. 30-35Artikkel i tidsskrift (Annet vitenskapelig)
  • 1055.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek: 12. Ekkrös. Exidia truncata2005Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, nr 20, s. 35-37Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1056.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek: 13. Limsvamp Bulgaria inquinans2006Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, nr 21, s. 21-24Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1057.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek: 14. Rostöra (Wood fungi on oak 14.-Oak Curtain Crust, Hymenochaete rubiginosa (Fr.) Lev2007Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, nr 22, s. 33-36Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1058.
    Sunhede, Stellan
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek: 15. Askticka (Perenniporia fraxinea)2008Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, nr 23, s. 31-34Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1059.
    Sunhede, Stellan
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Meiere, D
    Högskolan i Skövde, Institutionen för vård och natur.
    Vedsvampar på ek - 10: Ekskinn (Aleurodiscus disciformis)2003Inngår i: Ekbladet: medlemsskrift för Ekfrämjandet, ISSN 0283-4839, Vol. 18, s. 27-30Artikkel i tidsskrift (Annet (populærvitenskap, debatt, mm))
  • 1060.
    Sunhede, Stellan
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Vasiliauskas, Rimvydas
    Högskolan i Skövde, Institutionen för vård och natur.
    Hotade tickor på ek i Litauen [Threatened polypores on oak in Lithuania.]2003Inngår i: Svensk botanisk tidskrift, ISSN 0039-646X, Vol. 97, s. 252-265Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Rare and endangered species of Aphyllophorales have been studied on Quercus robur in Lithuania. In total, 5455 trees and 742 stumps of oaks have been investigated in pure oak stands, mixed deciduous woods, mixed coniferous–deciduous woods, parks, and in open landscapes. Fungi in the herbarium BILAS (Vilnius) were examined.

    The ecology, distribution, and status of the following polypores are treated, viz Fistulina hepatica (Schaeff.: Fr.) With., Grifola frondosa (Dicks.: Fr) S.F.Gray, Hapalopilus croceus (Pers.: Fr.) Bond. & Sing, Inocutis dryophila (Berk) Fiasson & Niemelä, Inonotus dryadeus (Pers.: Fr.) Murr., Piptoporus quercinus (Fr.) Pilát, and Polyporus umbellatus (Pers.) ex Fr. Each species is briefly described and localities are listed in detail. All species, except F. hepatica and P. quercinus, are included in the Lithuanian Red List of fungi. We suggest these two species should be added to the list, and classified according to the IUCN categories Near Threatened (NT) and Endangered (EN), respectively.

    The importance of saving the right type of substrate and habitat for the actual species is stressed. Thick dead, standing or fallen trees, and old stumps should not be removed, as F. hepatica, G. frondosa, H. croceus, and P. quercinus may continue to fructify for many years on this type of wood. Suppression of old host trees, formerly growing in a more open environment, is a problem at many sites. A proper clearing around these oaks is necessary to keep the oaks alive as long as possible. The necessity of favouring younger oaks of different age classes in the stands is pointed out, as a continuous supply of thick host trees is needed to preserve the fungi.

    Hapalopilus croceus, one of Europe’s most endangered fungi, fructifies on old thick oaks, a substrate that becomes more and more rare. All Lithuanian host trees of H. croceus are proposed to be preserved (even when dead) together with a sufficient number of oaks in the surroundings. In Sweden, which is still one of the strongholds of H. croceus in Europe, the species is classified as Critically Endangered (CR) and since 2000 also protected.

    The following two oak sites in Lithuania are highly recommended to be preserved, more closely investigated, and managed for the future: Punios Šilas (squares 31, 32 & 38), Alytus district. In this very valuable locality, where many old and thick oaks are suppressed by surrounding younger trees, we found H. croceus (on 11 oaks), I. dryophila, F. hepatica, P. quercinus, and the red-listed beetle Osmoderma eremita (Scop.). “Kauno Ažuolynas” in Kaunas is a unique park with more than 800 mostly old, thick oaks, mixed with, e.g., Tilia. Here we observed F. hepatica, G. frondosa (on 11 oaks), H. croceus (on 8 oaks), and I. dryophila.

  • 1061.
    Sutkowska, Agnieszka
    et al.
    University of Agriculture in Krakow, Poland.
    Pasierbinski, Andrzej
    University of Silesia in Katowice, Poland.
    Warzecha, Tomasz
    University of Agriculture in Krakow, Poland.
    Mandal, Abul
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Mitka, Jozef
    Jagiellonian University in Krakow, Poland.
    Refugial pattern of Bromus erectus in Central Europe based on ISSR fingerprinting: Phylogeography of Bromus erectus in Central Europe2013Inngår i: Acta Biologica Cracoviensia. Series Botanica, ISSN 0001-5296, E-ISSN 1898-0295, Vol. 55, nr 2, s. 107-119Artikkel i tidsskrift (Fagfellevurdert)
    Fulltekst (pdf)
    SSutkowska et al-2013
  • 1062.
    Svanström, Leif
    Högskolan i Skövde, Institutionen för vård och natur. Department of Public Health Sciences, Division of Social Medicine, Karolinska Institutet, Stockholm, Sweden.
    It all started in Falköping, Sweden: Safe Communities - global thinking and local action for safety2012Inngår i: International Journal of Injury Control and Safety Promotion, ISSN 1745-7300, E-ISSN 1745-7319, Vol. 19, nr 3, s. 202-208Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    After constructing the Safe Community model and applying it in Falkoping Municipality, Sweden, a first step was taken to establish a Swedish network for knowledge exchange between Safe Communities. Falkoping was the first to be involved, and was then joined by Lidkoping and Motala. Later, there followed Harstad in Norway, and some communities in Australia. Criteria were developed to define the concept operationally. In 1986, collaboration was embarked upon with the World Health Organization, and since 1991 there have been annual conferences on Safe Communities. Many academic centres around the world are now involved. Certification of communities started in 1989, and 272 communities have now been designated as a Safe Community (20 February 2011). The regional organisations, especially the Asian and European networks, embrace more than half of the designated communities. A global organisation has been considered, but the strength of the movement lies in local engagement and regional networking.

  • 1063.
    Svanström, Leif
    Högskolan i Skövde, Institutionen för vård och natur. Department of Public Health Sciences, Division of Social Medicine, Karolinska Institutet, Stockholm, Sweden.
    Professional autobiography of Professor Leif Svanström - with a focus on injury prevention and safety promotion2012Inngår i: International Journal of Injury Control and Safety Promotion, ISSN 1745-7300, E-ISSN 1745-7319, Vol. 19, nr 3, s. 198-201Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Professor Svanstrom has spent about forty-five years in the field of Social Medicine and Health and Safety Promotion. His main lines of research and teaching are Injury Epidemiology and Safety Promotion. In the 1960s, he conducted a number of descriptive and analytical studies, and in the 1970s began to address home and occupational injuries. In 1974, he introduced the community approach to safety promotion, encapsulated in the Falköping Model, which has heavily influenced Swedish and international community safety work. Under his leadership of the Research Group on Injury Prevention and Safety Promotion at the Karolinska Institutet in Stockholm, Sweden, more than 30 doctorates have been awarded. His work as Head of the WHO Collaborating Centre on Community Safety Promotion has led to the establishment of Safe Communities worldwide.

  • 1064.
    Svanström, Rune
    Högskolan i Skövde, Institutionen för vård och natur.
    När livsvärldens mönster brister2010Rapport (Annet (populærvitenskap, debatt, mm))
  • 1065.
    Svanström, Rune
    Högskolan i Skövde, Institutionen för vård och natur.
    När livsvärldens mönster brister: erfarenheter av att leva med demenssjukdom2009Doktoravhandling, monografi (Annet vitenskapelig)
    Abstract [en]

    This thesis is focusing on the lived experience of dementia. Both living with a partner as well as living alone. There is no unequivocal picture of how it is to live with dementia and few studies have been carried out in homes of afflicted persons. A deeper understanding of how it is to live with dementia can be a good starting-point for caring and the organisation of care.

    The aim of this thesis is to describe, clarify and explain the lived experience of dementia from a lifeworld theoretical point of view. Another aim is to illuminate how decision makers look upon persons with dementia, their life and their care. Interviews and observations have been used to collect data. Participating informants have been couples with one partner suffering from dementia, persons with dementia living alone, politicians, administrators and social workers. Data was analysed with a phenomenological and a hermeneutical approach.

    To live as a couple where one part has dementia (study 1) implies to live in a heteronomous existence where both the person with dementia and the partner become strangers in a world that should be the most well-known and familiar. The couple’s existence is narrowed and controlled by the impact of the dementia disease and the existence  is characterised of hopelessness and homelessness.

    To live alone with dementia (study 2) means to live with a broken identity when the person with dementia gradually loses the memory of himself and his life. It becomes a life where the world of the individual is reduced to a quiet background that does not demand attention. The person with dementia does not longer know how he or she should relate to the world. The existence is characterised by a strong sense of loneliness and only a vague knowledge of the situation. The person with dementia longs for other people and gets a sense of boredom in the existence.

    The comprehensive interpretation (study 3) shows that life with dementia is characterised by a gradual loss of meaning in life due to a disturbed intentionality. With disturbed intentionality the person with dementia gets increasingly more difficulties in understanding the meaning of the use of everyday objects. The person with dementia fights this and tries to create meaningfulness in the existence – something which gets very difficult and strenuous in time, since even the easiest everyday chores have to be thought through to make sense and even to be accomplished. The effort can in time become overpowering for the person with dementia who then stops doing the chores and becomes passive.

    Politicians, administrators and social workers (study 4) are well aware that dementia gives suffering to the afflicted and the partner. The care is not designed to meet their needs for home care, and the decision makers don’t know how to change this. The person with dementia becomes like an object when the social workers don’t includes them in a dialogue about their needs and care. The partner is left alone in solving difficult problems and in making difficult decisions.

    The theory of intentionality can help the professionals in the care of persons with dementia. By helping the cared-for-person to ‘stretch the intentional threads’ the caregivers can give the person a possibility to be rooted in the world. A care that supports intentionality and identity and reaches all the way in to the homes of the person with dementia would improve their situation and increase their well-being. This is possible when engaging the person in every-day chores that promote meaning. This kind of care contributes to the possibility for the person with dementia to be rooted in language, time and space.

  • 1066.
    Svanström, Rune
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson Sundler, Annelie
    Högskolan i Skövde, Institutionen för vård och natur. School of Health, Care and Social Welfare, Mälardalen University, Eskilstuna, Sweden.
    Berglund, Mia
    Högskolan i Skövde, Institutionen för vård och natur.
    Westin, Lars
    Högskolan i Skövde, Institutionen för vård och natur.
    Suffering caused by care - elderly patients’ experiences in community care2013Inngår i: International Journal of Qualitative Studies on Health and Well-being, ISSN 1748-2623, E-ISSN 1748-2631, Vol. 8, nr 1, artikkel-id 20603Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Growing old involves many changes in life and implies an increased risks of illness and different forms of disabilities. Life may change in a radical way when a person gets a disease like dementia or moves to a nursing home due to disabilities or needs. In both cases, it often leads to an increased dependency on care where the patient becomes exposed and vulnerable and thereby at a higher risk for experiencing different forms of suffering.

    Aim: The aim of this study was to elucidate and gain a deeper understanding of elderly patients’ experiences of suffering in relation to community care in nursing homes and home care services.

    Materials and methods: A lifeworld hermeneutical approach was used. Phenomenological interviews and conversations with an open approach were conducted and analysed with a focus on meanings.

    Findings: The findings were presented in four main themes; an absence of the other in care, an absence of dialogues, a sense of alienation and a sense of insecurity. The findings in this study revealed that persons who were cared for in nursing homes and home care services sometimes were exposed to an unnecessary suffering. The suffering sometimes was caused by various caring actions, that is, unnecessary suffering. The suffering caused by care that aroused was due to caregiver’s inability to be present, to show their face, and truly meet the patient.

    Conclusion: Suffering from care increased the elderly patients’ feelings of insecurity, loneliness, and alienation; this seemed to be the foundation for patients’ experiences of being outside a human community. There was a lack of knowledge and understanding about the patient’s lifeworld.

    Fulltekst (pdf)
    fulltext
  • 1067.
    Svantesson, Ulla
    et al.
    Institute of Neuroscience and Physiology, Sahlgren ska Academy, University of Gothenburg, Sweden.
    Zander, Martina
    Department of Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Sweden.
    Klingberg, Sofia
    Department of Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Sweden.
    Slinde, Frode
    Högskolan i Skövde, Institutionen för vård och natur.
    Body composition in male elite athletes, comparison of bioelectrical impedance spectroscopy with dual energy X-ray absorptiometry2008Inngår i: Journal of Negative Results in Biomedicine, ISSN 1477-5751, E-ISSN 1477-5751, Vol. 7, nr 1, s. 1-5, artikkel-id 1Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background

    The aim of this study was to compare body composition results from bioelectrical spectroscopy (BIS) with results from dual energy X-ray absorptiometry (DXA) in a population of male elite athletes. Body composition was assessed using DXA (Lunar Prodigy, GE Lunar Corp., Madison, USA) and BIS (Hydra 4200, Xitron Technologies Inc, San Diego, California, USA) at the same occasion. Agreement between methods was assessed using paired t-tests and agreement-plots.

    Results

    Thirty-three male elite athletes (soccer and ice hockey) were included in the study. The results showed that BIS underestimates the proportion of fat mass by 4.6% points in the ice hockey players. In soccer players the BIS resulted in a lower mean fat mass by 1.1% points. Agreement between the methods at the individual level was highly variable.

    Conclusion

    Body composition results assessed by BIS in elite athletes should be interpreted with caution, especially in individual subjects. BIS may present values of fat mass that is either higher or lower than fat mass assessed by DXA, independent of true fat content of the individual.

  • 1068.
    Svensson, Andreas
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Rydetorp, Sofia
    Högskolan i Skövde, Institutionen för vård och natur.
    Sjuksköterskors upplevelser av avlastningssamtal på akutmottagningar2009Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Sjuksköterskor inom akutsjukvården har ett varierande yrke. Ingen dag är den andra lik och av sjuksköterskor krävs snabba beslut och ett lugnande arbetssätt. I ett rum kan en patient vårdas för en fiskekrok i fingret medan patienten i rummet bredvid är döende. Detta kan göra att personalen mår dålig och blir stressad vilket kan leda till brister i omvårdnaden mot patienterna. Syftet med studien var att undersöka och beskriva sjuksköterskors upplevelser av avlastningssamtal. För att nå syftet gjordes en intervjustudie. Sju sjuksköterskor från tre olika akutmottagningar intervjuades. Graneheim och Lundmans (2004) analysmetod användes och intervjuerna resulterade i sex kategorier: Tillhörighet i arbetsgruppen, trivsel, stimulans, enkelhet, att växa i sin yrkesroll, tidslig och rumslig uppfattning. Avlastningssamtalen sker både informellt och formellt bland personalen. Arbetsgruppen har stor betydelse och ett bra klimat på arbetsplatsen bidrar till att underlätta spontana avlastningssamtal. För personal som varit med om samma händelser kan samtalet ge en ökad förståelse för var och ens insats och det bidrar till att sjusköterskor mognar och växer i sin yrkesroll. Det upplevs också som en trygghet och lättnad att känna förtroende för kollegorna och kunna prata om sådant som känns tungt. Det behövs mer forskning inom det här området särskilt vad sjuksköterskor har för behov av avlastande samtal.

    Fulltekst (pdf)
    FULLTEXT01
  • 1069.
    Svensson, Ann-Marie
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Bergh, Ingrid
    Högskolan i Skövde, Institutionen för vård och natur.
    Jakobsson, Eva
    Institute of Health and Care Sciences, The Sahlgrenska Academy, University of Gothenburg.
    Older Peoples' Descriptions of Becoming and Being Respite Care Recipients2011Inngår i: Journal of Housing for the Elderly, ISSN 0276-3893, E-ISSN 1540-353X, Vol. 25, s. 159-174Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The number of older people in the western world is increasing. Advancing age creates the need for care, including respite care. The aim of this study is to describe the experience of older people of becoming and being respite care recipients. Admission to respite care is a response to a range of practicalities in the home, such as the need to maintain privacy, dissatisfaction with home care, and deterioration in health. However, the participants in this study had little or no involvement in the decision regarding respite care placement. Activities, training, and medical treatment were found to be important to respite care recipients’ satisfaction with their care.

  • 1070.
    Svensson, Ann-Marie
    et al.
    School of Health Sciences, Jönköping University, Jönköping, Sweden.
    Mårtensson, Lena
    Högskolan i Skövde, Institutionen för vård och natur. College of Nursing, University of Rhode Island, USA.
    Hellström Muhli, Ulla
    Department of Sociology, Uppsala University, Sweden.
    Well-being dialogue: Elderly women’s subjective sense of well-being from their course of life perspective2012Inngår i: International Journal of Qualitative Studies on Health and Well-being, ISSN 1748-2623, E-ISSN 1748-2631, Vol. 7, s. Article Number: 19207-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this article, we are concerned with narratives of elderly women’s well-being from their perspectives of the latter parts of their life, living at special housing accommodation (SHA) in the context of Swedish elderly care. In focusing on narratives about well-being, we have a two-fold focus: (1) how the elderly women create their own identity and meaning-making based on lifetime experience; and (2) how narratives of well-being are reflected through the filter of life in situ at the SHA. Based on empirical data consisting of well-being narratives, a dialogical performance analysis was undertaken. The results show how relationships with important persons during various stages of life, and being together and enjoying fellowship with other people as well as enjoying freedom and self-determination, are central aspects of well-being. The conclusions drawn are that the characteristic phenomena of well-being (the what) in the narratives are continuity, identity, and sociality for the elderly person, and this is manifested (the how) as a question of contrasting the state of self-management and self-decline.

  • 1071.
    Svensson, Linda
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Vester, Malin
    Högskolan i Skövde, Institutionen för vård och natur.
    BVC-sjuksköterskors erfarenheter av att ge stöd till mödrar med post partum depression2012Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Bakgrund: En moder som lider av post partum depression har svårare att knyta an till sitt barn. Barnets kognitiva och känslomässiga utveckling kan då hämmas. Det är viktigt att tidigt upptäcka dessa mödrar för att på så vis kunna erbjuda stöd och förkorta moderns sjukdomstid. Syfte: Syftet var att belysa BVC-sjuksköterskors erfarenheter av att ge stöd till mödrar med post partum depression. Metod: Datamaterialet analyserades med kvalitativ innehållsanalys med induktiv ansats. Data insamlades genom intervjuer med åtta BVC-sjuksköterskor med specialistexamen som distriktssköterska verksamma inom barnhälsovård. Resultat: Ur analysen av datamaterialet framträdde sex kategorier så som; följsam till moderns behov, erbjuda råd och vägledning, bygga upp moderns insikt om post partum depression, initiera samarbete runt modern, känsla av egen otillräcklighet, kommunikationsproblem med 14 underkategorier. Konklusion: För att kunna erbjuda modern ett optimalt stöd behöver en relation byggd på förtroende skapas mellan BVC-sjuksköterskan och modern. Kommunikation är ett viktigt verktyg för att skapa tillit vilket är en förutsättning för relationen.

    Fulltekst (pdf)
    BVC-sjuksköterskors erfarenheter av att ge stöd till mödrar med post partum depression
  • 1072.
    Svensson, Maria
    Högskolan i Skövde, Institutionen för vård och natur.
    Studies of genes involved in regulating flowering time in Arabidopsis2006Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Transition from a vegetative growth phase to flowering in plants occurs in response to both environmental conditions and endogenous signals. Identification of genes that are involved in regulating the time of flowering is of great importance in agri- and horticulture. Flowering-time genes can be used for crop improvement by, for instance, engineering plants to flower earlier. This shortening of the time to flowering could result in an extended growing season that could enable farmers to grow more than one crop each year. In this work, a gene knockout approach using T-DNA tagging and in vivo gene fusion has been employed to identify and characterise genes that are involved in regulating flowering time in the model plant Arabidopsis thaliana. This approach resulted in the identification of two genes, At4g20010 and its homologue At1g31010. Expression studies and GUS histochemical analysis of a reporter gene revealed that At4g20010 is mainly expressed in rapid growing tissues such as root tips, shoot apex, flowers and stem nodes. T-DNA insertional mutants of At4g20010 and At1g31010 exhibit a late-flowering phenotype that can largely be repressed by application of gibberellin. Plants with an insertional mutation in At4g20010 contain a reduced amount of the bioactive gibberellin GA4 compared to wild-type plants. The decreased level of GA4 is not due to a transcriptional repression of the GA-biosynthetic genes AtGA3ox1 or AtGA20ox1, since their expressions were increased in the mutant plants. In silico analyses revealed that the C-terminal protein sequences encoded by At4g20010 and At1g31010 contain RNA-binding motifs, whereas the N-terminal sequences have three-dimensional structures similar to single stranded nucleic acid-binding proteins. To conclude, At4g20010 and At1g31010 may encode two RNA-binding proteins that are involved in regulating flowering time in A. thaliana by affecting the metabolism of GA. This can be possible either by a positive regulation of GA3ox at the post-transcriptional level or by a negative regulation of GA2ox.

  • 1073.
    Svensson, Maria
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Lundh, Dan
    Högskolan i Skövde, Institutionen för kommunikation och information.
    Bergman, Per
    Department of Plant Biology and Forest Genetics, SLU, SE-750 07 Uppsala, Sweden.
    Mandal, Abul
    Högskolan i Skövde, Institutionen för vård och natur.
    Characterisation of a T-DNA-tagged gene of Arabidopsis thaliana that regulates gibberellin metabolism and flowering time2005Inngår i: Functional Plant Biology, ISSN 1445-4408, E-ISSN 1445-4416, Vol. 32, nr 10, s. 923-932Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging. Plants containing a T-DNA insert in the 3′-UTR of At4g20010 flowered later under both long- and short-day conditions compared with control plants. Histochemical assays of the mutant plants showed that the promoterless gus gene is expressed predominantly in the shoot apex, but it is also expressed in root tips, stem nodes and in the abscission zone of developing siliques. Measurement of endogenous gibberellin (GA) showed that bioactive GA4 levels in mutant plants were reduced compared with wild type (WT) plants. Like other known mutants defective in GA biosynthesis, the late-flowering phenotype observed in our T-DNA-tagged line could be largely repressed by application of exogenous GA3. The T-DNA-tagged gene At4g20010 encodes a previously uncharacterised protein belonging to the DUF731 family. Sequence analysis showed similarity to a single-stranded binding domain and to an RNA-binding protein of Chlamydomonas reinhardtii. Considering the above results (sequence similarity, mutant phenotype and level of endogenous GA), we propose that At4g20010 is an RNA-binding protein involved in regulating GA biosynthesis, possibly at the post-transcriptional level.

  • 1074.
    Svensson, Maria
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Lundh, Dan
    Högskolan i Skövde, Institutionen för kommunikation och information.
    Ejdebäck, Mikael
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Mandal, Abul
    Högskolan i Skövde, Institutionen för vård och natur.
    Functional prediction of a T-DNA tagged gene of Arabidopsis thalianaby in silico analysis2004Inngår i: Journal of Molecular Modeling, ISSN 1610-2940, E-ISSN 0948-5023, Vol. 10, nr 2, s. 130-138Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We have employed a gene-knockout approach using T-DNA tagging and in vivo gene fusion in Arabidopsis thaliana for identification and isolation of specific plant genes. Screening of about 3,000 T-DNA tagged lines resulted in identification of a mutant line (no. 197) exhibiting a significant delay in flowering. From this line a 600-bp plant DNA fragment downstream of the left T-DNA junction was cloned by inverse PCR. BLAST searching in the A. thaliana genomic database indicated a putative gene, frf (flowering regulating factor), with unknown function downstream of the T-DNA insert. Bioinformatic tools were used to predict possible protein structure and function. The protein structure predicted by fold recognition indicates that frf is a transcriptional regulator, a ligand-binding receptor responsive to steroids and hormones. Analyzing the predicted results and the phenotype of the T-DNA tagged plant we hypothesized that FRF might be involved in hormone response in A. thaliana. For verification of this hypothesis we exposed the plants of line no. 197 to gibberellic acid (GA3), a potential growth regulator in higher plants. This treatment resulted in an earlier onset of flowering, almost similar to that in wild type control plants.

  • 1075.
    Svensson, Martin
    Högskolan i Skövde, Institutionen för vård och natur.
    Strukturer i grus- och sandtäkter som styr förekomsten av bibagge Apalus bimaculatus i Västra Götalands län2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Rapporten är en analys av inventeringsdata på bibagge (Apalus bimaculatus) och vårsidenbi (Colletes cunicularius) från Länsstyrelsen i Västra Götalands län. Naturvårdsverket har utformat ett åtgärdsprogram för bibagge då den är upptagen på rödlistan som nära hotad (NT). Bibaggen är en brandgul skalbagge som man finner i blottade, solexponerade, sandytor där dess värdart vårsidenbiet lever. Bibaggen är nämligen boparasit på vårsidenbiet. Endast under parningstiden, några få veckor i slutet av mars och början av april, är bibaggen aktiv ovan mark. Detta är anledningen till att den för bara några år sedan var en förbisedd art i den svenska skalbaggsfaunan. Idag finns arten främst i grus- och sandtäkter men då man nu övergår till att använda sig av bergkross istället för sand från täkter riskerar dess livsmiljöer att växa igen och försvinna. I rapporten analyseras inventeringsdata med utgångspunkt på att hitta de strukturer i täkterna som styr förekomsten av bibagge och vårsidenbi. Analyserna gjordes med hjälp av regressionsträd, korrelationer och t-test. Resultatet blev som förväntat att den viktigaste förklaringsfaktorn till att det fanns bibaggar i en täkt var att det fanns vårsidenbibon. Vid framtida inventeringar för att finna denna svårinventerade art kan det vara en bra ide att koncentrera sig på att leta efter vårsidenbibon för att på ett effektivare sätt hitta lämpliga lokaler för bibagge.

    Fulltekst (pdf)
    fulltext
  • 1076.
    Svensson, Susanne
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Johansson, Elaine
    Högskolan i Skövde, Institutionen för vård och natur.
    Omvårdnad av diabetespatienter med annan kulturell bakgrund än sjuksköterskans: en litteraturöversikt2009Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    Samhället blir mer och mer mångkulturellt och diabetes är en folksjukdom som blir alltvanligare. Sjuksköterskan vårdar patienter med olika kulturer och religioner och ställs då införutmaningar som att överbrygga språkproblem och göra sig förstådd, samt att anpassaomvårdnad av diabetes efter patientens kultur. Syftet med studien var att utifrån litteraturenbelysa omvårdanden av diabetespatienter med annan kulturell bakgrund än sjuksköterskansutifrån sjuksköterskans och patientens perspektiv. Med en litteraturstudie av vetenskapligaartiklar läggs en grund för framtida fortsatt forskning inom området. Totalt nio vetenskapligaartiklar analyserades och fyra områden som författarna ansåg vara relevanta till syftet medstudien framkom: Hur påverkar patientens kultur upplevelsen av diabetes och omvårdnad,traditioner och diabetes, sjuksköterskans roll i omvårdnaden av diabetes patienter med annankulturell bakgrund och kommunikation mellan sjuksköterskan och patienten. Resultatet visadeatt det är av stor vikt att sjuksköterskan är medveten om omfattningen av kulturella skillnadersamt att vården individanpassas efter patientens önskemål och behov.

    Fulltekst (pdf)
    FULLTEXT01
  • 1077.
    Swärdh, Anna
    Högskolan i Skövde, Institutionen för vård och natur.
    Fibromyalgi: En litteraturstudie om fibromyalgins möjliga riskfaktorer2013Independent thesis Basic level (university diploma), 5 poäng / 7,5 hpOppgave
    Abstract [sv]

    Bakgrund: Fibromyalgi är ett syndrom som kännetecknas av utbredd smärta,

    överkänslighet vid beröring men även att övriga delar av kroppen påverkas.

    Metod: En litteraturstudie har genomförts där 10 stycken vetenskapliga artiklar har

    analyserats och utgjort resultatet.

    Syfte: Huvudsyftet är att undersöka möjliga riskfaktorer som kan påverka utvecklandet

    av fibromyalgi.

    Resultat: Studiens resultat har tagit upp fyra teman med tänkabara riskfaktorer.

    Traumatiska barndomshändelser, där både fysisk och verbal misshandel och sexuellt

    utnyttjande var riskfaktorer. Att bli utsatt för trauma som vuxen, där fysisk misshandel,

    operation och arbetsrelaterad skada ingick, var också en riskfaktor. Stress och ett högt

    BMI-värde var också riskfaktorer relaterade till utvecklandet av fibromyalgi.

    Diskussion: Studiens resultat visade på att det är flera riskfaktorer som påverkar

    utvecklandet av detta syndrom. Alla som utsätts för en av dessa riskfaktorer utvecklar

    dock inte fibromyalgi, utan med största sannolikhet så är det en kombination av flera

    riskfaktorer som gör att man utvecklar fibromyalgi.

    Slutsats: Det behövs mer forskning för att studera sambandet mellan fysiska, psykiska

    och sociala riskfaktorer.

    Fulltekst (pdf)
    fulltext
  • 1078.
    Synnergren, Jane
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Identification of miRNAs in Control of Aberrant Gene Transcription in Human Pluripotent Stem Cell Derived Hepatocytes2013Inngår i: Computer Applications in Industry and Engineering (CAINE-2013), 26th International Conference September 25-27, 2013, Los Angeles, California, USA / [ed] Sultan Aljahdali, International Society for Computers and Their Applications , 2013, s. 11-16Konferansepaper (Fagfellevurdert)
  • 1079.
    Synnergren, Jane
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    MicroRNA regulatory network involved in impaired functionality in cardiomyocytes derived from human embryonic stem cells2012Inngår i: 25th International Conference on Computer Applications in Industry and Engineering 2012 (CAINE-2012) Held with the 4th International Symposium on Sensor Network and Application (SNA-2012): New Orleans, Louisiana, USA 14-16 November 2012 / [ed] Gongzhu Hu, International Society for Computers and Their Applications , 2012, s. 133-138Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Human embryonic stem cells (hESCs) have unique properties of proliferation and self-renewal, and can be differentiated into various functional cell types e.g. cardiomyocytes. However, previous studies have shown that the expression of cardiac ion channels and genes involved in the Ca2+-handling machinery is immature in the stem cell derived cardiomyocytes, and novel approaches are therefore needed to improve the differentiation protocols and produce more functional cardiomyocytes. MicroRNAs (miRNAs) are small molecules, which play key roles in regulation of cellular development and may therefore be powerful tools to improve the differentiation.

    This paper presents a method to derive a miRNA-mRNA regulatory network, which likely are important for the regulation of the functionality that currently is lacking in the hESC-derived cardiomyocytes. In total 14 ion channels and 9 calcium handling genes that have important roles in cardiac tissue and which have shown to be significantly lower expressed in hESC-derived cardiomyocytes compared to their in vivo counterpart, were investigated and scanned for putative miRNA target sites. For each of the predicted miRNAs, a combined prediction score (CPS) was calculated and a miRNA regulatory network was generated consisting of miRNAs with a high CPS and with multiple targets among the investigated genes. Results from this study propose that the miRNA network presented here is highly involved in the hampered functionality seen in hESC-derived cardiomyocytes, and that it therefore will constitute an important tool to select candidate miRNAs for future knockout- and overexpression studies.

  • 1080.
    Synnergren, Jane
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Transcriptional profiling of human embryonic stem cells and their functional derivatives2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Human    embryonic    stem    cells    (hESCs)    represent    populations    of    pluripotent, undifferentiated  cells  with  unlimited  replication  capacity,  and  with  the  ability  to differentiate into any functional cell type in the human body. Based on these properties, hESCs  and  their  derivatives  provide  unique  model  systems  for  basic  research  on embryonic development. Also, industrial in vitro applications of hESCs are now beginning to  find  their  way  into  the  fields  of  drug  discovery  and  toxicology.  Moreover,  hESC-derivatives are anticipated to be promising resources for future cell replacement therapies. However, in order to fully utilize the potential of hESCs it is necessary to increase our knowledge about the processes that govern the differentiation of these cells. At present, some  of  the  major  challenges  in  stem  cell  research  are  heterogeneous  cell  populations, insufficient  yield  of  the  differentiated  cell  types  and  immature  derivatives  with  limited functionality.  To  address  these  problems,  a  better  understanding  of  the  regulatory mechanisms  that  control  the  lineage  commitment  is  needed.  The  aim  of  this  thesis  has been to increase the knowledge of the global transcriptional programs which are activated when  cells  differentiate  along  specific  pathways,  and  to  identify  key  genes  that  show differential expression at specific stages of differentiation. The results indicate that hESCs express a unique set of housekeeping genes that are stably expressed in this specific cell type  and  in  their  derivatives,  which  highlights  the  importance  of  proper  validation  of reference genes for usage in hESCs. Furthermore, an extensive characterization of hESCs and differentiated progenies of the cardiac and hepatic lineages has been conducted, and sets  of  differentially  expressed  genes  were  identified.  Two  different  protocols,  which mediate  definitive  and  primitive  endoderm  respectively,  were  studied,  and  important discrepancies  between  these  two  cell  types  were  identified.  Moreover,  the  global expression profile of hESC-derived cardiomyocyte clusters were thoroughly investigated and compared to that of foetal and adult heart. To further study regulatory mechanisms of  importance  during  stem  cell  differentiation,  the  global  expression  of  microRNAs (miRNAs) was also investigated. Putative target genes of differentially expressed miRNAs were  identified  using  computational  predictions,  and  their  mRNA  expression  was analysed. Notably, an interesting correlation between the miRNA and mRNA expression was observed, which supports the general notion that miRNAs bind to and degrade their target mRNAs, and thus act as fine-tuning regulators of gene expression. Taken together, the results described in this thesis provide important information for further studies on regulatory mechanisms that control the differentiation of hESCs into functional cell types such as cardiomyocytes and hepatocytes. 

  • 1081.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Adak, Sudeshna
    GE John F Welch Technol Ctr Export Promot Ind Pk, Bangalore, Karnataka, India.
    Englund, Mikael
    Celllartis AB, SE-43146 Gothenburg, Sweden.
    Giesler, Theresa
    GE Healthcare, Piscataway, NJ 08855 USA.
    Noaksson, Karin
    Celllartis AB, SE-43146 Gothenburg, Sweden.
    Lindahl, Anders
    Sahlgrens Univ Hosp, Dept Clin Chem Transfus Med, SE-41345 Gothenburg, Sweden.
    Nilsson, Patric
    Högskolan i Skövde, Institutionen för vård och natur.
    Nelson, Deirdre
    GE Global Res Ctr, Moscow 123098, Russia.
    Abbot, Stewart
    GE Global Res Ctr, Moscow 123098, Russia.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för vård och natur.
    Sartipy, Peter
    Celllartis AB, SE-43146 Gothenburg, Sweden.
    Cardiomyogenic gene expression profiling of differentiating human embryonic stem cells2008Inngår i: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 134, nr 1-2, s. 162-170Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Human embryonic stem cells (hESCs) can differentiate into a variety of specialized cell types. Thus, they provide a model system for embryonic development to investigate the molecular processes of cell differentiation and lineage commitment. The development of the cardiac lineage is easily detected in mixed cultures by the appearance of spontaneously contracting areas of cells. We performed gene expression profiling of undifferentiated and differentiating hESCs and monitored 468 genes expressed during cardiac development and/or in cardiac tissue. Their transcription during early differentiation of hESCs through embryoid bodies (EBs) was investigated and compared with spontaneously differentiating hESCs maintained on feeders in culture without passaging (high-density (HD) protocol). We observed a larger variation in the gene expression between cells from a single cell line that were differentiated using two different protocols than in cells from different cell lines that were cultured according to the same protocol. Notably, the EB protocol resulted in more reproducible transcription profiles than the HD protocol. The results presented here provide new information about gene regulation during early differentiation of hESCs with emphasis on the cardiomyogenic program. In addition, we also identified regulatory elements that could prove critical for the development of the cardiomyocyte lineage.

  • 1082.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi. Department of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital.
    Ameen, Caroline
    Cellartis, Göteborg, Sweden.
    Jansson, Andreas
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sartipy, Peter
    Cellartis, Göteborg, Sweden.
    Global transcriptional profiling reveals similarities and differences between human stem cell-derived cardiomyocyte clusters and heart tissue2012Inngår i: Physiological Genomics, ISSN 1094-8341, E-ISSN 1531-2267, Vol. 44, nr 4, s. 245-258Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    It is now well documented that human embryonic stem cells (hESCs) can differentiate into functional cardiomyocytes. These cells constitute a promising source of material for use in drug development, toxicity testing, and regenerative medicine. To assess their utility as replacement or complement to existing models, extensive phenotypic characterization of the cells is required. In the present study, we used microarrays and analyzed the global transcription of hESC-derived cardiomyocyte clusters (CMCs) and determined similarities as well as differences compared with reference samples from fetal and adult heart tissue. In addition, we performed a focused analysis of the expression of cardiac ion channels and genes involved in the Ca2+-handling machinery, which in previous studies have been shown to be immature in stem cell-derived cardiomyocytes. Our results show that hESC-derived CMCs, on a global level, have a highly similar gene expression profile compared with human heart tissue, and their transcriptional phenotype was more similar to fetal than to adult heart. Despite the high similarity to heart tissue, a number of significantly differentially expressed genes were identified, providing some clues toward understanding the molecular difference between in vivo sourced tissue and stem cell derivatives generated in vitro. Interestingly, some of the cardiacrelated ion channels and Ca2+-handling genes showed differential expression between the CMCs and heart tissues. These genes may represent candidates for future genetic engineering to create hESC-derived CMCs that better mimic the phenotype of the cardiomyocytes present in the adult human heart.

  • 1083.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Améen, Caroline
    Cellartis, Gothenburg, Sweden.
    Lindahl, Anders
    Dept of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital, Sweden.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sartipy, Peter
    Cellartis, Gothenburg, Sweden .
    Expression of microRNAs and their target mRNAs in human stem cell-derived cardiomyocyte clusters and in heart tissue2011Inngår i: Physiological Genomics, ISSN 1094-8341, E-ISSN 1531-2267, Vol. 43, nr 10, s. 581-594Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Recent studies have shown that microRNAs (miRNAs) act as posttranscriptional regulators and that they play important roles during heart development and in cardiac function. Thus, they may provide new means of altering stem cell fate and differentiation processes. However, information about the correlation between global miRNA and mRNA expression in cardiomyocyte clusters (CMCs) derived from human embryonic stem cells (hESC) and in fetal and adult heart tissue is lacking. In the present study the global miRNA and mRNA expression in hESC-derived CMCs and in fetal and adult heart tissue was investigated in parallel using microarrays. Target genes for the differentially expressed miRNAs were predicted using computational methods, and the concordance in miRNA expression and mRNA levels of potential target genes was determined across the experimental samples. The biology of the predicted target genes was further explored regarding their molecular functions and involvement in known regulatory pathways. A clear correlation between the global miRNA expression and corresponding target mRNA expression was observed. Using three different sources of cardiac tissue-like samples, we defined the similarities between in vitro hESC-derived CMCs and their in vivo counterparts. The results are in line with previously reported observations that miRNAs repress mRNA expression and additionally identify a number of novel miRNAs with potential important roles in human cardiac tissue. The concordant miRNA expression pattern observed among all the cardiac tissue-like samples analyzed here provide a starting point for future ambitious studies aiming towards assessment of the functional roles of specific miRNAs during cardiomyocyte differentiation.

  • 1084.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för kommunikation och information.
    Giesler, Therese L.
    GE Healthcare, Piscataway, NJ, United States.
    Adak, Sudeshna
    GE John F. Welch Technology Centre Export Promotion Industrial Park, Bangalore, India.
    Tandon, Reeti
    GE John F. Welch Technology Centre Export Promotion Industrial Park, Bangalore, India.
    Noaksson, Karin
    Cellartis AB, Göteborg, Sweden.
    Lindahl, Anders
    Department of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital, Göteborg, Sweden.
    Nilsson, Patric
    Högskolan i Skövde, Institutionen för vård och natur.
    Nelson, Deirdre
    GE Global Research Center, Niskayuna, NY, United States.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för kommunikation och information.
    Englund, Mikael C. O.
    Cellartis AB, Göteborg, Sweden.
    Abbott, Stewart
    GE Global Research Center, Niskayuna, NY, United States.
    Sartipy, Peter
    Cellartis AB, Göteborg, Sweden / Cellartis AB, Arvid Wallgrens Backe 20, SE-41346 Göteborg, Sweden.
    Differentiating human embryonic stem cells express a unique housekeeping gene signature2007Inngår i: Stem Cells, ISSN 1066-5099, E-ISSN 1549-4918, Vol. 25, nr 2, s. 473-480Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Housekeeping genes (HKGs) are involved in basic functions needed for the sustenance of the cell and are assumed to be constitutively expressed at a constant level. Based on these features, HKGs are frequently used for normalization of gene expression data. In the present study, we used the CodeLink Gene Expression Bioarray system to interrogate changes in gene expression occurring during differentiation of human ESCs (hESCs). Notably, in the three hESC lines used for the study, we observed that the RNA levels of 56 frequently used HKGs varied to a degree that rendered them inappropriate as reference genes. Therefore, we defined a novel set of HKGs specifically for hESCs. Here we present a comprehensive list of 292 genes that are stably expressed (coefficient of variation <20%) in differentiating hESCs. These genes were further grouped into high-, medium-, and low-expressed genes. The expression patterns of these novel HKGs show very little overlap with results obtained from somatic cells and tissues. We further explored the stability of this novel set of HKGs in independent, publicly available gene expression data from hESCs and observed substantial similarities with our results. Gene expression was confirmed by real-time quantitative polymerase chain reaction analysis. Taken together, these results suggest that differentiating hESCs have a unique HKG signature and underscore the necessity to validate the expression profiles of putative HKGs. In addition, this novel set of HKGs can preferentially be used as controls in gene expression analyses of differentiating hESCs.

  • 1085.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Heins, Nico
    Cellartis AB, Göteborg.
    Brolén, Gabriella
    Cellartis AB, Göteborg.
    Eriksson, Gustav
    Cellartis AB, Göteborg.
    Lindahl, Anders
    Sahlgrenska University Hospital.
    Hyllner, Johan
    Cellartis AB, Göteborg.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sartipy, Peter
    Cellartis AB, Göteborg.
    Björnquist, Petter
    Cellartis AB, Göteborg.
    Transcriptional profiling of human embryonic stem cells differentiating to definitive and primitive endoderm and further towards the hepatic lineage2010Inngår i: Stem Cells and Development, ISSN 1547-3287, E-ISSN 1557-8534, Vol. 19, nr 7, s. 961-978Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Human embryonic stem cells (hESC) can differentiate into a variety of specialized cell types, and they constitute a useful model system to study embryonic development in vitro. In order to fully utilize the potential of these cells, the mechanisms that regulate the developmental processes of specific lineage differentiation need to be better defined. The aim of this study was to explore the molecular program involved in the differentiation of hESC towards definitive endoderm (DE) and further into the hepatic lineage, and to compare that to primitive endoderm (PrE) differentiation. To that end, we applied two protocols, a specific DE differentiation protocol and an intrinsic differentiation protocol that mainly mediates PrE formation. We collected hESC, hESC-derived DE, DE-derived hepatocyte-progenitors (DE-Prog), DE-derived hepatocyte-like cells (DE-Hep), and the corresponding PrE-derivatives. The samples were analyzed using microarrays, and we identified sets of genes which were exclusively up-regulated in DE-derivatives (compared to PrE-derivatives) at discrete developmental stages. We also investigated known protein interactions among the set of up-regulated genes in DE-Hep. The results demonstrate important differences between DE- and PrE-differentiation on the transcriptional level. In particular, our results identify a unique molecular program, exclusively activated during development of DE and the subsequent differentiation of DE towards the hepatic lineage. We identified key-genes and pathways of potential importance for future efforts to improve hepatic differentiation from hESC. These results reveal new opportunities for rational design of specific interventions with the purpose of generating enriched populations of DE derivatives, including functional hepatocytes.

    Fulltekst (pdf)
    Synnergren2010
  • 1086.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Olsson, Björn
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Gamalielsson, Jonas
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    A Data Integration Method for Exploring Gene Regulatory Mechanisms2008Inngår i: Conference on Information and Knowledge Management: Proceedings of the 2nd international workshop on Data and text mining in bioinformatics, ACM Press, 2008, s. 81-84Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Systems biology aims to understand the behavior of and interaction between various components of the living cell, such as genes, proteins, and metabolites. A large number of components are involved in these complex systems and the diversity of relationships between the components can be overwhelming, and there is therefore a need for analysis methods incorporating data integration. We here present a method for exploring gene regulatory mechanisms which integrates various types of data to assist the identification of important components in gene regulation mechanisms. By first analyzing gene expression data, a set of differentially expressed genes is selected. These genes are then further investigated by combining various types of biological information, such as clustering results, promoter sequences, binding sites, transcription factors and other previously published information regarding the selected genes. Inspired by Information Fusion research, we also mapped functions of the proposed method to the well-known OODA-model to facilitate application of this data integration method in other research communities. We have successfully applied the method to genes identified as differentially expressed in human embryonic stem cells at different stages of differentiation towards cardiac cells. We identified 15 novel motifs that may represent important binding sites in the cardiac cell linage.

  • 1087.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Olsson, Björn
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Gamalielsson, Jonas
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Classification of information fusion methods in systems biology2009Inngår i: In Silico Biology, ISSN 1386-6338, Vol. 9, nr 3, s. 65-76Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Biological systems are extremely complex and often involve thousands of interacting components. Despite all efforts, many complex biological systems are still poorly understood. However, over the past few years high-throughput technologies have generated large amounts of biological data, now requiring advanced bioinformatic algorithms for interpretation into valuable biological information. Due to these high-throughput technologies, the study of biological systems has evolved from focusing on single components (e.g. genes) to encompassing large sets of components (e.g. all genes in an entire genome), with the aim to elucidate their interdependences in various biological processes. In addition, there is also an increasing need for integrative analysis, where knowledge about the biological system is derived by data fusion, using heterogeneous data sets as input. We here review representative examples of bioinformatic methods for fusion-oriented interpretation of multiple heterogeneous biological data, and propose a classification into three categories of tasks that they address: data extraction, data integration and data fusion. The aim of this classification is to facilitate the exchange of methods between systems biology and other information fusion application areas.

    Fulltekst (pdf)
    fulltext
  • 1088.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Sartipy, Peter
    Cellartis AB, Sweden.
    Microarray Analysis of Undifferentiated and Differentiated Human Pluripotent Stem Cells2011Inngår i: Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications / [ed] Craig Atwood, Rijeka, Croatia: INTECH, 2011, s. 343-366Kapittel i bok, del av antologi (Fagfellevurdert)
  • 1089.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Sartipy, Peter
    Cellartis AB, Sweden.
    Transcriptional analysis of messenger-RNA and micro-RNA array data reveals global negative correlation in human stem cell derived cardiomyocyte clusters2012Inngår i: 4th International Conference on Bioinformatics and Computational Biology / [ed] F. Saeed H. Al-Mubaid, Curran Associates, Inc., 2012, s. 178-183Konferansepaper (Fagfellevurdert)
    Abstract [en]

    MicroRNAs (miRNAs) are small non-coding molecules that have been shown to play key roles in regulating cellular development and to be involved in various diseases. By interfering with their target mRNAs, these molecules inhibit the expression of proteins, either by de-stabilizing the messenger RNA (mRNA) molecule or by preventing its translation. Each miRNA can target hundreds of mRNAs, and one mRNA can be targeted by several miRNAs.This makes it extremely complex to determine the regulatory functions of specific miRNAs in the transcription and translation processes. However, important advancements in microarray technology have made large scale monitoring of miRNA expression possible. This opens the possibility to move from studies of single molecules to studies of complex molecular processes, pathways, and biological systems. Recent studies have indicated important roles for miRNAs in stem cell differentiation in general and in cardiac development in particular. This paper presents a global transcriptional study where putativecorrelation between miRNA and mRNA expression in fetal- and adult heart tissues samples and in clusters of cardiomyocytes derived from human embryonic stem cells (hESCs) were investigated. Target genes of miRNAs expressed in cardiac tissue have been predicted and their transcriptional profiles investigated in tissue specimen. By using a statistical algorithm, up- and down-regulated miRNAs and mRNAs have been identified in four different cardiac related samples. In total, 17 cardiac related miRNAs have been analyzed and for each of these, clusters of negatively correlated target genes were identified. This supports the hypothesis that sets of miRNAs play an important role in modulating the expression of genes, which are important for cardiac development. Interestingly, the results from this study also indicate a global negative correlation between miRNA expression and mRNA expression, which previously has not been reported.

  • 1090.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Åkesson, Karolina
    Cellartis AB, Gothenburg, Sweden.
    Dahlenborg, Kerstin
    Cellartis AB, Gothenburg, Sweden.
    Vidarsson, Hilmar
    Cellartis AB, Gothenburg, Sweden.
    Ameen, Caroline
    Cellartis AB, Gothenburg, Sweden.
    Steel, Daniella
    Cellartis AB, Gothenburg, Sweden.
    Lindahl, Anders
    Sahlgrens Univ Hosp, Dept Clin Chem Transfus Med, S-41345 Gothenburg, Sweden.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för vård och natur.
    Sartipy, Peter
    Cellartis AB, Gothenburg, Sweden.
    Molecular signature of cardiomyocyte clusters derived from human embryonic stem cells2008Inngår i: Stem Cells, ISSN 1066-5099, E-ISSN 1549-4918, Vol. 26, nr 7, s. 1831-1840Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Human embryonic stem cells (hESCs) can differentiate in vitro into spontaneously contracting cardiomyocytes (CMs). These cells may prove extremely useful for various applications in basic research, drug discovery, and regenerative medicine. To fully use the potential of the cells, they need to be extensively characterized, and the regulatory mechanisms that control hESC differentiation toward the cardiac lineage need to be better defined. In this study, we used microarrays to analyze, for the first time, the global gene expression profile of isolated hESC-derived CM clusters. By comparing the clusters with undifferentiated hESCs and using stringent selection criteria, we identified 530 upregulated and 40 downregulated genes in the contracting clusters. To further characterize the family of upregulated genes in the hESC-derived CM clusters, the genes were classified according to their Gene Ontology annotation. The results indicate that the hESC-derived CM clusters display high similarities, on a molecular level, to human heart tissue. Moreover, using the family of upregulated genes, we created protein interaction maps that revealed topological characteristics. We also searched for cellular pathways among the upregulated genes in the hESC-derived CM clusters and identified eight significantly upregulated pathways. Real-time quantitative polymerase chain reaction and immunohistochemical analysis confirmed the expression of a subset of the genes identified by the microarrays. Taken together, the results presented here provide a molecular signature of hESC-derived CM clusters and further our understanding of the biological processes that are active in these cells.

  • 1091.
    Synnergren, Jane
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Özdogan, Alper
    Yildiz Technical University, Turkey.
    Olsson, Björn
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Sartipy, Peter
    Cellartis AB, Göteborg, Sweden.
    Clustering micro-RNA array data using an information fusion based approach with multiple types of input data2010Inngår i: Proceedings of the ISCA 2nd International Conference on Bioinformatics and Computational Biology, BICoB-2010, March 24-26, 2010, Sheraton Waikiki Hotel, Honolulu, Hawaii, USA / [ed] Hisham Al-Mubaid, International Society for Computers and Their Applications , 2010, s. 151-158Konferansepaper (Fagfellevurdert)
    Abstract [en]

    MicroRNAs (miRNAs) are small non-coding molecules that have been shown to play key roles in regulating cellular development and to be involved in various diseases. By interfering with their target mRNAs, these molecules inhibit the expression of proteins, either by destabilizing the mRNA molecule or by preventing its translation. It has been suggested that each miRNA can target hundreds of mRNAs, and that one mRNA can be targeted by several miRNAs. This makes it extremely complex to determine the roles of specific miRNAs in the regulation of translation of mRNA. Recent advancements in microarray technology have made large-scale monitoring of miRNA expression possible. However, the size and complexity of these data sets make them challenging to analyze, and improved algorithms are therefore required to facilitate the analysis. In this paper, we present a novel clustering algorithm that uses an Information Fusion (IF) approach to cluster miRNA data, allowing for multiple types of input data to guide the clustering. For evaluation of the algorithm, we used miRNA expression data from human embryonic stem cells and cardiomyocyte-like cells derived thereof. Clusters obtained when using the multiple input data approach were compared to those generated when using only the expression data. Our results show that it is beneficial to include various types of genomic data as input to the clustering process, since it results in clusters of increased biological relevance.

  • 1092.
    Söderström, Josefina
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Mattsson, Emma
    Högskolan i Skövde, Institutionen för vård och natur.
    Sjuksköterskestudenters uppfattningar om sin framtida roll som arbetsledare: En intervjustudie2009Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

     

    Ledarskapet är en del av sjuksköterskans arbete och ingår i sjuksköterskans profession. Sjuksköterskestudenterna behöver vara förberedda för rollen som arbetsledare. Syftet med studien är att beskriva sjuksköterskestudenters uppfattningar om sin framtida yrkesroll som arbetsledare. Datamaterialet består av intervjuer med tio sjuksköterskestudenter som studerar på en högskola i Västsverige.  Åldern på sjuksköterskestudenterna varierar mellan 22 och 38 år. Intervjuerna spelades in på band och transkriberades. En kvalitativ analysmetod valdes för att analysera insamlad data. Efter genomförd analys kunde fyra huvudkategorier urskiljas. Huvudkategorierna är sjuksköterskans ansvar, samarbeta, förberedelse och personlig lämplighet. Under dessa huvudkategorier framkom åtta underkategorier. Resultatet visar att sjuksköterskestudenterna upplever att de inte är tillräckligt förberedda för rollen som arbetsledare. Sjuksköterskestudenterna anser att sjuksköterskans ansvar innebär att ge information och undervisning till patienter, studenter och kollegor samt att leda och fördela arbetet. Sjuksköterskestudenterna upplever att samarbete innebär att arbeta tillsammans med alla yrkeskategorier, vara flexibel samt att verka för en god kommunikation i arbetslaget. De upplever att de saknar ledarskapsutbildning och pedagogiska kunskaper. Sjuksköterskestudenterna anser att personlig lämplighet påverkar ledarskapsrollen.

    Fulltekst (pdf)
    FULLTEXT02
  • 1093.
    Sögaard, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Szekeres, Ferenc
    Department of Molecular Medicine and Surgery, Section og Integrative Physiology, Karolinska Institutet.
    Holmström, Maria
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Larsson, Dennis
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Harlén, Mikael
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Garcia-Roves, Pablo
    Section of Integrative Physiology, Department of Physiology and Pharmacology, Karolinska Institutet.
    Chibalin, Alexander V.
    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet.
    Effects of fibre type and diffusion distance on mouse skeletal muscle glycogen content in vitro2009Inngår i: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 107, nr 6, s. 1189-1197Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In vitro incubation of isolated rodent skeletal muscle is a widely used procedure in metabolic research. One concern with this method is the development of an anoxic state during the incubation period that can cause muscle glycogen depletion. Our aim was to investigate whether in vitro incubation conditions influence glycogen concentration in glycolytic extensor digitorum longus (EDL) and oxidative soleus mouse muscle. Quantitative immunohistochemistry was applied to assess glycogen content in incubated skeletal muscle. Glycogen concentration was depleted, independent of insulin-stimulation in the incubated skeletal muscle. The extent of glycogen depletion was correlated with the oxidative fibre distribution and with the induction of hypoxia-induced-factor-1-alpha. Insulin exposure partially prevented glycogen depletion in soleus, but not in EDL muscle, providing evidence that glucose diffusion is not a limiting step to maintain glycogen content. Our results provide evidence to suggest that the anoxic milieu and the intrinsic characteristics of the skeletal muscle fibre type play a major role in inducing glycogen depletion in during in vitro incubations.

  • 1094.
    Sögård, Peter
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur.
    Mathematical Modelling of Insulin Signalling: Effects on Glucose Metabolism in Skeletal Muscle2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The use of models to understand complex phenomena is indispensable to the scientific community. The advantage of a model is that it simplifies the phenomena under study. However, a model should be only as complex as required, no more, no less. Furthermore, a model should avoid known or unknown confounding variables that might obscure the interpretations of observations. Within biology, models can be set up in many different ways, such as mathematical, graphical or verbal descriptions of the system under study. In physiology, the systems under study can be the entire animal or organs or cell cultures from it. To study some aspects of the regulation of glucose and energy homeostasis, skeletal muscles is a preferable model, as it is the main consumer of post-prandial glucose, and thus, important for maintaining whole body glucose and energy homeostasis. Incubation of skeletal muscle specimens in a suitable solution is a model-system that has been used during the last century. The availability of oxygen for energy transformation has been of major concern. Therefore, the experimental system has been validated several times with different methods, both experimentally and mathematically.

    The result from experimental validations indicates that glycogen content is unequally distributed within the incubated muscle specimens, with the core depleted of glycogen. Furthermore, validation done with the mathematical models describing the experimental systems indicates that oxygen diffusion is sufficient if the following assumptions are valid; homogeneous structure and that the critical value of oxygen pressure is above zero throughout the entire muscle. However, if those assumptions are invalid, the observations of some metabolic and/or signalling data might be invalid. In this thesis, those assumption are validated, with the specific aim to derive mathematical models that can be used to further analyse the metabolic data generated.

    Set of ordinary differential equation was used to describe the metabolic data derived from incubation of mouse extensor digitorum longus skeletal muscles preparations, paper 1. The parameters and constants were identified within the mathematical model, which then, was further analysed. The results indicated that the experimental system suffered from anoxia and that glycogen was depleted during the incubation time. An immunohistochemical approach was used to verify the predictions from the mathematical model on glycogen depletion, paper 2. A statistical approach was developed herein that made quantitative studies possible and the results verified the prediction from the mathematical model in paper 1. Furthermore, a correlation between fibre type distribution and glycogen depletion was observed, indicating that the assumption on homogeneous glucose handling might be too hard. The existence of anoxia within the incubated muscle specimens was revealed. A novel hypothesis regarding deficient insulin diffusion into the centre of the incubated muscle preparation as the cause for quasi-depletion of glycogen was tested, paper 3. The hypothesis was falsified; instead increased insulin signalling was observed in the core of the muscle, correlating with fibre types on the single-cell-level.

    In conclusion, the studies presented in this thesis provide evidence that muscle preparations are suffering of anoxia after incubation leading to depletion of glycogen. Furthermore, the assumption on homogeneous glucose handling is falsified. Finally, a mathematical model is provided that can be used to estimate the un-measurable glycogen concentrations and estimate the glucose uptake rate in the superficial fibres.

  • 1095.
    Sögård, Peter
    et al.
    Högskolan i Skövde, Forskningscentrum för Systembiologi. Högskolan i Skövde, Institutionen för vård och natur. Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Harlén, Mikael
    Högskolan i Skövde, Institutionen för vård och natur.
    Long, Yun Chau
    Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Szekeres, Ferenc
    Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Barnes, Brian R.
    Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Chibalin, Alexander V.
    Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Zierath, Juleen R.
    Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
    Validation of the in vitro incubation of extensor digitorum longus muscle from mice with a mathematical model2010Inngår i: Journal of biological systems, ISSN 0218-3390, Vol. 18, nr 3, s. 687-707Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In vitro incubation of tissues; in particular, skeletal muscles from rodents, is a widely-used experimental method in diabetes research. This experimental method has previously been validated, both experimentally and theoretically. However, much of the method's experimental data remains unclear, including the high-rate of lactate production and the lack of an observable increase in glycogen content, within a given time. The predominant hypothesis explaining the high-rate of lactate production is that this phenomenon is dependent on a mechanism in glycolysis that works as a safety valve, producing lactate when glucose uptake is super-physiological. Another hypothesis is that existing anoxia forces more ATP to be produced from glycolysis, leading to an increased lactate concentration. The lack of an observable increase in glycogen content is assumed to be dependent on limitations in sensitivity of the measuring method used. We derived a mathematical model to investigate which of these hypotheses is most likely to be correct. Using our model, data analysis indicates that the in vitro incubated muscle specimens, most likely are sensing the presence of existing anoxia, rather than an overflow in glycolysis. The anoxic milieu causes the high lactate production. The model also predicts an increased glycogenolysis. After mathematical analyses, an estimation of the glycogen concentration could be made with a reduced model. In conclusion, central anoxia is likely to cause spatial differences in glycogen concentrations throughout the entire muscle. Thus, data regarding total glycogen levels in the incubated muscle do not accurately represent the entire organ. The presented model allows for an estimation of total glycogen, despite spatial differences present in the muscle specimen.

  • 1096.
    Sögård, Peter
    et al.
    Högskolan i Skövde, Institutionen för vård och natur. Department of Surgical Sciences, Karolinska Institute, Stockholm, Sweden.
    Harlén, Mikael
    Högskolan i Skövde, Institutionen för vård och natur.
    Svensson, L. T.
    Arexis AB, Gothenburg, Sweden.
    Zierath, J. R.
    Department of Surgical Sciences, Karolinska Institute, Stockholm, Sweden.
    Nilsson, Patric
    Högskolan i Skövde, Institutionen för vård och natur.
    Integration of mathematical and experimental approaches to resolve insulin signaling2005Inngår i: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 183, nr 1, s. 125-126Artikkel i tidsskrift (Fagfellevurdert)
  • 1097.
    Tanveer, M. K.
    et al.
    Högskolan i Skövde, Institutionen för vård och natur.
    Kamran, A.
    Department of Parasitology, University of Veterinary and animal Sciences, Lahore, Pakistan.
    Abbas, Muhammad Athar
    Högskolan i Skövde, Institutionen för vård och natur.
    Umer, Nighat C.
    Department of Parasitology, University of Veterinary and animal Sciences, Lahore, Pakistan / Department of comparative biology and experimental medicine, University of Calgary, AB T2N 1N4, Canada.
    Azhar, M. A.
    Department of Parasitology, University of Veterinary and animal Sciences, Lahore, Pakistan.
    Munir, Muhammad Arif
    Department of Biomedical Sciences and Veterinary Public Health, Division of Virology, Research and Development, Department of Virology, Immunobiology and Parasitology of the National, Veterinary Institute (SVA) and Swedish University of Agricultural Sciences (SLU), Ulls väg 2B, SE-751 89 Uppsala, Sweden.
    Prevalence and chemo-therapeutical investigations of gastrointestinal nematodes in domestic pigeons in Lahore, Pakistan2011Inngår i: Tropical Biomedicine, ISSN 0127-5720, Vol. 28, nr 1, s. 102-110Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The prevalence of gastrointestinal nematodes was studied in 143 (80 male and 63 female) domestic pigeons. Faecal samples were collected to determine the gastrointestinal nematodes of domestic pigeons through qualitative and quantitative faecal examinations. A total of 48 (male 33 and 25 female) naturally infected domestic pigeons were divided into G(1) (albendzdole) and G(2) (fenbendazole) treatment-groups along with one control group (C). The overall prevalence of gastrointestinal nematodes was 40.5% (58/143) in domestic pigeons. Likewise, the prevalence of gastrointestinal nematodes in males and females was found 41.3% (33/58) and 39.7% (25/58) respectively. The overall prevalence of Capillaria obsignata and Ascaridia columbae was found to be 67.2% and 32.8%, respectively. The prevalence of C. obsignata and A. columbae in males was 72.7% (24/33) and 27.8% (9/33) and in females was 60% (15/25) and 40% (10/25), respectively. There was no significant sex related difference seen in the prevalence of C. obsignata (p>0.56) and A. columbae (p>0.40) in domestic pigeons, respectively. The overall efficacy of albendazole and fenbendazole was calculated to be 66% and 71%. A remarkable significant difference (p<0.05) was observed in eggs per gram before and after treatment in both G(1) and G(2) treated-groups. The efficacy of fenbendazole was found to be more significant (p<0.02) than albendazole.

  • 1098.
    Teder, Marie
    et al.
    Linköping University, Sweden.
    Morelius, Evalotte
    Linköping University, Sweden.
    Nordwall, Maria
    Linköping University, Sweden / Vrinnevi Hospital, Norrköping, Sweden.
    Bolme, Per
    Linköping University, Sweden / Vrinnevi Hospital, Norrköping, Sweden.
    Ekberg, Joakim
    Högskolan i Skövde, Institutionen för vård och natur.
    Wilhelm, Elisabeth
    Linköping University, Sweden.
    Timpka, Toomas
    Linköping University, Sweden.
    Family-Based Behavioural Intervention Program for Obese Children: An Observational Study of Child and Parent Lifestyle Interpretations2013Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, nr 8, artikkel-id e71482Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Family-based behavioural intervention programs (FBIPs) against childhood obesity have shown promising results, but the mediating mechanisms have not been identified. The aim of this study was to examine changes in obese childrens lifestyle habits during a 2-year FBIP according to their own and parents' reports, the concordance between these reports and the correlations to change in post-intervention z-BMI. Methods: An observational study of 26 children (8.3-12.0 years) and their parents participating in a 2-year FBIP was performed. Weight and height were measured from baseline to 12 months after the end of the program. Eating habits and physical-and sedentary activity were reported separately by children and parents. Data were analysed with regard to concordance between parents' and children's reports and association between the lifestyle reports and change in z-BMI at the study endpoint using descriptive statistics and parametric and non-parametric tests. Results: According to both children's and parents' reports, the level of physical activity among the children had increased after the intervention as well as the agreement between the informants' reports. According to the children, eating habits had improved, while the parents' reports showed an improvement only with regard to binge eating. The concordance between children and parents regarding eating habits was slight to fair also after the intervention. No statistically significant associations between changes in lifestyle reports and changes in z-BMI were observed. Conclusions: Child and parent reports of physical activity were found to converge and display an improvement in a 2-year FBIP, while the reports on eating habits showed a more refractory pattern. Changes in concordance and agreement between children and parents reports did not correlate with weight reduction. Further methods development and studies of the processes during family-based interventions against childhood obesity are warranted.

  • 1099.
    Thernström Blomqvist, Ylva
    et al.
    Uppsala University.
    Rubertsson, Christine
    Uppsala University.
    Kylberg, Elisabeth
    Högskolan i Skövde, Institutionen för vård och natur.
    Jöreskog, Karin
    Uppsala University.
    Hedberg, Kerstin
    Uppsala University.
    Kangaroo Mother Care helps fathers of preterm infants gain confidence in the paternal role2012Inngår i: Journal of Advanced Nursing, ISSN 0309-2402, E-ISSN 1365-2648, Vol. 68, nr 9, s. 1988-1996Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aim.  This article is a report on a descriptive study of fathers’ experiences of providing their preterm infants with Kangaroo Mother Care.

    Background.  During neonatal intensive care, fathers describe the incubator as a barrier and the separation from their infant as stressful. Fathers consider it important to be close to the infant, and performing Kangaroo Mother Care makes them feel an important participant in their infants’ care.

    Method.  Individual interviews conducted in 2009 with seven fathers who performed Kangaroo Mother Care were analysed using qualitative content analysis.

    Results.  The fathers’ opportunity for being close to their infants facilitated attainment of their paternal role in the neonatal intensive care unit. Kangaroo Mother Care allowed them to feel in control and that they were doing something good for their infant, although the infant’s care could be demanding and stressful. As active agents in their infant’s care, some fathers stayed with the infant during the whole hospital stay, others were at the neonatal intensive care unit all day long. Despite the un-wished-for situation, they adapted to their predicament and spent as much time as possible with their infants.

    Conclusion.  Fathers’ opportunities for Kangaroo Mother Care helped them to attain their paternal role and to cope with the unexpected situation. The physical environment and conflicting staff statements influenced their opportunity for, and experience of, caring for their preterm infants.

  • 1100.
    Thorsell, K. B. E.
    et al.
    Section of Nursing, Department of Health Sciences, Faculty of Medicine, Lund University, Sweden / Section of Elderly, Municipality of Hässleholm, Sweden.
    Nordström, B. M.
    Section of Nursing, Department of Health Sciences, Faculty of Medicine, Lund University, Sweden.
    Fagerström, Lisbeth
    Högskolan i Skövde, Institutionen för vård och natur. Department of Health Sciences, Buskerud University College, Drammen, Norway.
    Sivberg, B. V.
    Section of Nursing, Department of Health Sciences, Faculty of Medicine, Lund University, Sweden.
    Time in Care for Older People Living in Nursing Homes2010Inngår i: Nursing Research and Practice, ISSN 2090-1429, E-ISSN 2090-1437, s. Article ID 148435, 10 pages-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In order to measure actual care needs in relation to resources required to fulfill these needs, an instrument (Time in Care) with which to evaluate care needs and determine the time needed for various care activities has been developed with the aim of assessing nursing intensity in municipal care for older people. Interreliability (ICC=0.854) of time measurements (n = 10´546) of 32 nursing activities in relation to evaluated care levels in two nursing homes (staff n = 81) has been determined. Nursing intensity for both periods at the two nursing homes comprised on average a direct care time of 75 (45%) and 101 (42%) minutes, respectively. Work time was measured according to actual schedule (462 hours per nursing home during two weeks). Given that the need for care was high, one must further investigate if the quality of care the recipients received was sufficiently addressed.

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