his.sePublications
Change search
Refine search result
1 - 1 of 1
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Rows per page
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sort
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
Select
The maximal number of hits you can export is 250. When you want to export more records please use the Create feeds function.
  • 1.
    Backlöf, Kalle
    University of Skövde, School of Life Sciences.
    GROWTH CONDITIONS AND EXPERIMENTAL SETUP FOR BACTERIAL GROWTH AND THE FADING OF PHENOLPHTHALEIN IN ALKALINE SOLUTION2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The whole project consisted of two different parts. The first subproject were running optical density measurements to assist in finding optimal growth conditions and experimental setup for use of E.coli BL21 (DE3) bacteria with the PYCARD gene transformed into them. The transformed bacteria will be used for generating data for modeling. Previous laboratory attempts had problems with timing of the exponential phase when several tests were performed simultaneously. The optimizations of a method for displaying growth include trying out using different medium but also different inoculation ratios between culture and medium to see the effects on growth rate. Results have shown that lysogeny broth together with an inoculation ratio of 1:25 results in rapid absorbance increase in the bacterial growth curves.The second part of the thesis project was to study the kinetics of fading of phenolphthalein in sodium hydroxide solution in order to provide a basis for experiments illustrating this in laboratory environment. Phenolphthalein is used in many different applications such as an active ingredient in some laxatives but the perhaps most common use is as an acid-base indicator of pH. The experiments were performed by mixing alkaline solutions of varying sodium hydroxide concentration together with sodium chloride, then after addition of phenolphthalein to study absorbance variations over time to illustrate the rate law which correlates the rate of color fade to sodium hydroxide concentration. The results have proven that they correspond directly so that increasing the concentration of sodium hydroxide raised the color fading rate just as much in percent.

1 - 1 of 1
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf