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  • 1.
    Ahmed, Bulbul
    et al.
    Rajshahi University, Rajshahi, Bangladesh.
    Khatun, Mansura
    Rajshahi University, Rajshahi, Bangladesh.
    Hossain, Monzur
    Rajshahi University, Rajshahi, Bangladesh.
    Islam, Rafiul
    Rajshahi University, Rajshahi, Bangladesh.
    Biswas, Manosh
    Rajshahi University, Rajshahi, Bangladesh.
    Mandal, Abul
    University of Skövde, School of Life Sciences. University of Skövde, The Systems Biology Research Centre.
    Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh2011In: Journal of Central European Agriculture, ISSN 1332-9049, E-ISSN 1332-9049, Vol. 12, no 3, p. 519-528Article in journal (Refereed)
    Abstract [en]

    Arabidopsis thaliana is a small flowering plant belonging to the Brassicaceae family, which is adopted as a model plant for genetic research. Agrobacterium tumifaciensmediated transformation method for A. thaliana ecotype Bangladesh was established. Leaf discs of A. thaliana were incubated with A. tumefaciens strain LBA4404 containing chimeric nos. nptII. nos and intron-GUS genes. Following inoculation and co-cultivation, leaf discs were cultured on selection medium containing 50 mg/l kanamycin + 50 mg/l cefotaxime + 1.5 mg/l NAA and kanamycin resistant shoots were induced from the leaf discs after two weeks. Shoot regeneration was achieved after transferring the tissues onto fresh medium of the same combination. Finally, the shoots were rooted on MS medium containing 50 mg/l kanamycin. Incorporation and expression of the transgenes were confirmed by PCR analysis. Using this protocol, transgenic A. thaliana plants can be obtained and indicates that genomic transformation in higher plants is possible through insertion of desired gene. Although Agrobacterium mediated genetic transformation is established for A. thaliana, this study was the conducted to transform A. thaliana ecotype Bangladesh.

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