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Non-Invasive Acoustical sensing of Drug-Induced Effects on the Contractile Machinery of Human Cardiomyocyte Clusters
Department of Applied Physics, Chalmers University of Technology, Göteborg, Sweden / Institute of Physical Chemistry, University of Göttingen, Göttingen, Germany.
Cellectis AB, Göteborg, Sweden / Abcam, Cambridge, United Kingdom.
Cellectis AB, Göteborg, Sweden.
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Cellectis AB, Göteborg, Sweden / AstraZeneca R&D, Mölndal, Sweden. (Bioinformatik, Bioinformatics)
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2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 5, 1-10 p., e0125540Article in journal (Refereed) Published
Abstract [en]

There is an urgent need for improved models for cardiotoxicity testing. Here we propose acoustic sensing applied to beating human cardiomyocyte clusters for non-invasive, surrogate measuring of the QT interval and other characteristics of the contractile machinery. In experiments with the acoustic method quartz crystal microbalance with dissipation monitoring (QCM-D), the shape of the recorded signals was very similar to the extracellular field potential detected in electrochemical experiments, and the expected changes of the QT interval in response to addition of conventional drugs (E-4031 or nifedipine) were observed. Additionally, changes in the dissipation signal upon addition of cytochalasin D were in good agreement with the known, corresponding shortening of the contraction-relaxation time. These findings suggest that QCM-D has great potential as a tool for cardiotoxicological screening, where effects of compounds on the cardiomyocyte contractile machinery can be detected independently of whether the extracellular field potential is altered or not.

Place, publisher, year, edition, pages
PLOS , 2015. Vol. 10, no 5, 1-10 p., e0125540
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:his:diva-12034DOI: 10.1371/journal.pone.0125540ISI: 000354542500033PubMedID: 25961711ScopusID: 2-s2.0-84930616030OAI: oai:DiVA.org:his-12034DiVA: diva2:911906
Funder
Knowledge Foundation
Available from: 2016-03-14 Created: 2016-03-14 Last updated: 2016-06-08Bibliographically approved

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Sartipy, Peter
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