his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana exhibit reduced accumulation of arsenics and increased tolerance to arsenate
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Högskolan i Skövde. (Bioteknik, Biotechnology)ORCID iD: 0000-0002-6319-4055
2015 (English)In: Transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana exhibit reduced accumulation of arsenics and increased tolerance to arsenate, Omics International Conference , 2015, Vol. 2, 32- p.Conference paper, (Refereed)
Abstract [en]

Transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana exhibit reduced accumulation of arsenics and increased tolerance to arsenate.

Toxic metals such as arsenics, lead, cadmium or chromium are the major environmental pollutants that severely contribute to contamination of the global food chain directly through their accumulation in the edible parts of the cultivated crops or indirectly via meat-milk pathway. Fortunately, plant genetic engineering has the potential for developing new crop cultivars for removal of the toxic substances from the polluted sources or for avoiding accumulation of these contaminants in the edible parts. Previously, we have identified and studied four key genes that are involved in accumulation of arsenics in plants (J Biol. Systems 2010, 18/1:1-19; J Mol. Modeling 2012, DOI 10.1007/s00894-012-1419 and J Mol. Modeling 2014, DOI 10.1007/s00894-014-2104). In this study, we have cloned and transformed the ACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into tobacco plants (Nicotiana tabacum). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild-type control plants. These plants can grow on medium containing 200 µM arsenate, whereas the non-transgenic plants can hardly survive under this condition. Furthermore, when exposed to 100 µM arsenate for 35 days accumulation of arsenics in shoots of the transgenic plants decreases significantly (28 µg/g d wt.) compared to that observed in the non-transgenic control plants (40 µg/g d wt.). This study shows that A. thaliana ACR2 gene is a potential candidate for genetic engineering of plants to develop new cultivars that can be grown on arsenic contaminated fields and can supply harmless foods containing no or significantly reduced amount of arsenics.

Place, publisher, year, edition, pages
Omics International Conference , 2015. Vol. 2, 32- p.
Series
Journal of Plant Physiology and Pathology, ISSN 2329-955X ; 2:5
Keyword [en]
Arsenic contamination, Human health, Phytoremediation, Transgenic plants
National Category
Natural Sciences Biological Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-11637DOI: 10.4172/2329-955X.C1.002OAI: oai:DiVA.org:his-11637DiVA: diva2:865723
Conference
Global Summit on Plant Science, September 21-23, 2015, San Antonio, Texas, USA
Projects
Bioremediation of toxic metals
Funder
Sida - Swedish International Development Cooperation Agency, AKT-2010-18
Note

 

Available from: 2015-10-29 Created: 2015-10-29 Last updated: 2016-01-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textAbstractAbstract (PDF)

Search in DiVA

By author/editor
Mandal, Abul
By organisation
School of BioscienceThe Systems Biology Research Centre
Natural SciencesBiological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 584 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf