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Expression of PDGF receptors and ligand-induced migration of partially differentiated human monocyte-derived macrophages. Influence of IFN-gamma and TGF-beta
Wallenberg Laboratory for Cardiovascular Research, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.ORCID iD: 0000-0002-4583-9315
Wallenberg Laboratory for Cardiovascular Research, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.
Wallenberg Laboratory for Cardiovascular Research, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.
Wallenberg Laboratory for Cardiovascular Research, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.
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2001 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 156, no 2, 267-275 p.Article in journal (Refereed) Published
Abstract [en]

In the early atherosclerotic lesion, monocytes accumulate at sites of inflammation and endothelial injury. Platelet-derived growth factor (PDGF), produced for example by macrophages, is a chemoattractant for smooth muscle cells and possibly also for macrophages. During early differentiation into macrophages, human monocytes (early hMDM) showed lower expression of PDGF alpha-receptor (PDGF-Ralpha) than beta-receptor (PDGF-Rbeta) mRNA. Early hMDM showed increased random motility (chemokinesis) in the presence of PDGF of the long (BB(L)) but not short (BB(S)) B-chain homodimer. Neither PDGF-AA(S) nor PDGF-AA(L) affected early hMDM motility. Since increased cytokine levels accompany inflammation, the influence of interferon-gamma (IFN-gamma) and transforming growth factor-beta (TGF-beta) on PDGF-R expression and migratory response were studied. Only PDGF-Ralpha mRNA was highly upregulated by IFN-gamma. TGF-beta only had minor effects on receptor mRNAs. Upregulation of PDGF-Ralpha levels by IFN-gamma was accompanied by significantly increased migration (chemotaxis) towards PDGF-AA(L) only. Consequently, IFN-gamma modulates PDGF-Rs expression in early hMDM and, subsequently, the chemotactic activity of PDGF-AA(L) on IFN-gamma-stimulated early hMDM. This suggests that PDGF-AA(L) may be involved in attracting activated monocytes to sites of inflammation and injury.

Place, publisher, year, edition, pages
Elsevier, 2001. Vol. 156, no 2, 267-275 p.
Keyword [en]
Cytokines, Macrophages, Migration, PDGF, Receptor
National Category
Other Basic Medicine
Research subject
Medical sciences
Identifiers
URN: urn:nbn:se:his:diva-11409DOI: 10.1016/S0021-9150(00)00644-4ISI: 000169476700003PubMedID: 11395022ScopusID: 2-s2.0-0034995007OAI: oai:DiVA.org:his-11409DiVA: diva2:848235
Available from: 2015-08-24 Created: 2015-08-24 Last updated: 2016-04-12Bibliographically approved

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Krettek, Alexandra
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