his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Characterisation and application of antibodies specific for the long platelet-derived growth factor A and B chains
Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Departamento de Biologı́a Celular, Universitat de Barcelona, Barcelona, Spain.
UPR9021 C.N.R.S., Immunologie et Chimie Thérapeutiques, I.B.M.C., Strasbourg, France.
Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.ORCID iD: 0000-0002-4583-9315
Show others and affiliations
2004 (English)In: International Journal of Biochemistry and Cell Biology, ISSN 1357-2725, E-ISSN 1878-5875, Vol. 36, no 11, 2226-2241 p.Article in journal (Refereed) Published
Abstract [en]

The platelet-derived growth factor (PDGF) family comprises important mitogens for mesenchymal cells. The active dimeric form of PDGF consists of four structurally related A, B, C, and D chains. All PDGF-variants bind to PDGF-receptors. The A and B chains occur with and without basic C-terminal amino acid extensions as long (A(L) and B(L)) and short (A(S) and B(S)) isoforms. PDGF-A and -B form homo- or heterodimers. The biological relevance of short and long isoforms is unknown, although it may relate to different affinities for glycosaminoglycans of the cell glycocalix and intercellular matrix. Commercially available anti-PDGF-A and anti-PDGF-B antibodies cannot discriminate between the short and the long isoforms. Thus, to investigate the function of the long and short isoforms, we raised antibodies specific for the long A and B chain isoforms. The antibodies were affinity-purified and their properties analysed by surface plasmon resonance. Inhibition studies with different PDGF homodimers and dot-blot studies proved their high specificity for the respective isoforms. Both antibodies recognised the target PDGF homodimers complexed to the glycocalix of human arterial smooth muscle cells and human monocyte-derived macrophages. By using these specific antibodies, we were able to confirm at the protein level the synthesis of PDGF-A and -B during differentiation of human monocyte-derived macrophages and to demonstrate the presence of the PDGF-A(L) and PDGF-B(L) isoforms in human arterial tissue.

Place, publisher, year, edition, pages
Elsevier, 2004. Vol. 36, no 11, 2226-2241 p.
Keyword [en]
GAG, glycosaminoglycans, hASMC, hMDM, human arterial smooth muscle cells, human monocyte-derived macrophages, LMW, low molecular weight, mean fluorescence intensity, MFI, PDGF, PDGF isoforms, platelet-derived growth factor, resonance units, RU
National Category
Other Basic Medicine
Research subject
Medical sciences
Identifiers
URN: urn:nbn:se:his:diva-11389DOI: 10.1016/j.biocel.2004.05.001ISI: 000223833700015PubMedID: 15313468Scopus ID: 2-s2.0-4143146480OAI: oai:DiVA.org:his-11389DiVA: diva2:847241
Available from: 2015-08-19 Created: 2015-08-19 Last updated: 2016-04-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Krettek, Alexandra
In the same journal
International Journal of Biochemistry and Cell Biology
Other Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 420 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf