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The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm.ORCID iD: 0000-0003-4221-6013
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia.
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia.
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2003 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 185, no 3, 843-853 p.Article in journal (Refereed) Published
Abstract [en]

The Escherichia coli BarA and UvrY proteins were recently demonstrated to constitute a novel two-component system, although its function has remained largely elusive. Here we show that mutations in the sensor kinase gene, barA, or the response regulator gene, uvrY, in uropathogenic E. coli drastically affect survival in long-term competition cultures. Using media with gluconeogenic carbon sources, the mutants have a clear growth advantage when competing with the wild type, but using media with carbon sources feeding into the glycolysis leads to a clear growth advantage for the wild type. Results from competitions with mutants in the carbon storage regulation system, CsrA/B, known to be a master switch between glycolysis and gluconeogenesis, led us to propose that the BarA-UvrY two-component system controls the Csr system. Taking these results together, we propose the BarA-UvrY two-component system is crucial for efficient adaptation between different metabolic pathways, an essential function for adaptation to a new environment.

Place, publisher, year, edition, pages
American Society for Microbiology , 2003. Vol. 185, no 3, 843-853 p.
National Category
Other Medical Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-10421DOI: 10.1128/JB.185.3.843-853.2003ISI: 000180834300016PubMedID: 12533459ScopusID: 2-s2.0-0037309144OAI: oai:DiVA.org:his-10421DiVA: diva2:772708
Available from: 2014-12-17 Created: 2014-12-17 Last updated: 2014-12-19Bibliographically approved

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Pernestig, Anna-Karin
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