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Electron transfer in ruthenium-modified spinach plastocyanin mutants
Department of Biochemistry and Biophysics, Lundberg Laboratory, Göteborg University / Chalmers University of Technology, Göteborg, Sweden.
Department of Biochemistry and Biophysics, Lundberg Laboratory, Göteborg University / Chalmers University of Technology, Göteborg, Sweden.ORCID iD: 0000-0002-3053-4543
Department of Organic Chemistry, Chalmers University of Technology, Göteborg, Sweden.
Department of Biochemistry and Biophysics, Lundberg Laboratory, Göteborg University / Chalmers University of Technology, Göteborg, Sweden.
1998 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, E-ISSN 1096-0384, Vol. 351, no 2, 197-206 p.Article in journal (Refereed) Published
Abstract [en]

Four site-directed mutants of spinach plastocyanin, Pc(Leu12His), Pc(Leu15His), Pc(Thr79His), and Pc(Lys81His), have been modified by covalent attachment of a photoactive [Ru(bpy)2(im)]2+ complex at the surface-exposed histidine residues. The Pc-Ru complexes were characterized with optical absorption, CD, and EPR spectroscopy and their spectra were found to be similar to the unmodified proteins except in the case of the Pc(Leu12His) mutant which lost the Cu ion irreversibly during the Ru modification. Electron transfer (ET) within the other Pc-Ru complexes was studied with time-resolved optical spectroscopy, using an external-quencher approach. The fully reduced [Cu(I), Ru(II)] proteins were photoexcited and subsequently oxidized by an external quencher, [Ru(NH3)6]Cl3, forming the [Cu(I), Ru(III)] proteins. This was followed by an internal ET from Cu(I) to Ru(III). The rates of the internal ET reactions exhibit an exponential dependence on metal-to-metal separation, with a decay factor of 1.1 A-1. From a temperature-dependence study of the Ru-modified Pc(Lys81His) protein, a reorganization energy for the Cu-to-Ru ET reaction of 1.2 eV was determined. In this analysis it was found necessary to include an appreciable temperature dependence in the driving force of the ET reaction.

Place, publisher, year, edition, pages
Academic Press, 1998. Vol. 351, no 2, 197-206 p.
National Category
Biochemistry and Molecular Biology
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-9901DOI: 10.1006/abbi.1997.0542ISI: 000072712900008PubMedID: 9514646Scopus ID: 2-s2.0-0032520654OAI: oai:DiVA.org:his-9901DiVA: diva2:743796
Available from: 2014-09-05 Created: 2014-09-05 Last updated: 2014-11-19Bibliographically approved

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