Biosorption of nickel by Lysinibacillus sp. BA2 native to bauxite mine
2014 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 107, 260-268 p.Article in journal (Refereed) Published
The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (−39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 min with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirmed by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.
Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 107, 260-268 p.
Lysinibacillus sp. BA2, Heavy metals, Biosorption, Adsorption isotherm
Biological Sciences Natural Sciences
Research subject Natural sciences
IdentifiersURN: urn:nbn:se:his:diva-9704DOI: 10.1016/j.ecoenv.2014.06.009ISI: 000342122000036PubMedID: 25011123ScopusID: 2-s2.0-84903900011OAI: oai:DiVA.org:his-9704DiVA: diva2:735945
FunderSida - Swedish International Development Cooperation Agency