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Biosorption of nickel by Lysinibacillus sp. BA2 native to bauxite mine
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, India.
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, India.
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, India.
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. (Physiology, Pharmacology and Toxicology)
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2014 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 107, 260-268 p.Article in journal (Refereed) Published
Abstract [en]

The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (−39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 min with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirmed by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 107, 260-268 p.
Keyword [en]
Lysinibacillus sp. BA2, Heavy metals, Biosorption, Adsorption isotherm
National Category
Biological Sciences Natural Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-9704DOI: 10.1016/j.ecoenv.2014.06.009ISI: 000342122000036PubMedID: 25011123ScopusID: 2-s2.0-84903900011OAI: oai:DiVA.org:his-9704DiVA: diva2:735945
Projects
Bioremediation
Funder
Sida - Swedish International Development Cooperation Agency
Available from: 2014-08-04 Created: 2014-08-04 Last updated: 2016-09-07Bibliographically approved
In thesis
1. Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Open this publication in new window or tab >>Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2016. 80 p.
Series
Örebro Studies in Life Science, ISSN 1653-3100 ; 15
Keyword
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
National Category
Other Biological Topics
Identifiers
urn:nbn:se:his:diva-12885 (URN)978-91-7529-146-8 (ISBN)
External cooperation:
Public defence
2016-09-22, Hus G, sal G 111, Högskolevägen, Skövde, 13:15 (English)
Opponent
Supervisors
Available from: 2016-09-07 Created: 2016-09-07 Last updated: 2016-09-08Bibliographically approved

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