his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High level of p37δ-mRNA relative to p110δ-mRNA in neuroblastoma tumors correlates with poor patient survival
Sahlgrenska Cancer Center, University of Gothenburg, Sweden.
University of Skövde, School of Life Sciences. University of Skövde, The Systems Biology Research Centre. Sahlgrenska Cancer Center, University of Gothenburg, Sweden.ORCID iD: 0000-0001-8962-0860
2013 (English)In: Medical Oncology, ISSN 1357-0560, E-ISSN 1559-131X, Vol. 30, no 4, 724Article in journal (Refereed) Published
Abstract [en]

Alterations in the PI3K/Akt pathway, a pathway that promotes proliferation and oncogenic transformation, are common in various cancers. In neuroblastoma, activation of Akt is correlated with aggressive disease although mutations in genes of this pathway are rare. Previous findings include a few mutations in PIK3CD, the gene encoding PI3K catalytic subunit delta, p110delta. We recently reported that an alternatively spliced form of p110delta, called p37delta, had cell proliferative properties and was over-expressed in ovarian and colorectal tumors. Here, we investigated p37delta in neuroblastoma primary tumors of different stages using qPCR (TaqMan) for gene expression analysis (46 samples) and Western blot for protein analysis (22 samples). Elevated levels of both p37delta-mRNA and p110delta-mRNA were detected in metastasizing neuroblastoma tumors compared to normal adrenal gland (P<0.05), and higher expression of p37delta-mRNA relative to p110delta-mRNA in neuroblastoma non-survivor patients compared to survivors (P<0.01). p37delta-Protein levels but not p110delta levels correlated with increased pAKT(T308) and pERK levels. The p37delta-mRNA levels did not correlate with the protein levels, indicating major regulation at the translational/protein level. Deregulation of signaling pathways is a hallmark of cancer development. Here, we show that p37delta, a kinase-dead isoform of the PI3K catalytic subunit p110delta, is over-expressed in neuroblastoma tumors, and that it correlates with the activation of both PI3K/Akt- and RAS-signaling pathways.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2013. Vol. 30, no 4, 724
Keyword [en]
p110d, p37d, Neuroblastoma, PI3K
National Category
Natural Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-8596DOI: 10.1007/s12032-013-0724-3ISI: 000327858800039PubMedID: 24026661Scopus ID: 2-s2.0-84883626316OAI: oai:DiVA.org:his-8596DiVA: diva2:660653
Note

Alternativ titel: High level of p37delta-mRNA relative to p110delta-mRNA in neuroblastoma tumors correlates with poor patient survival

Available from: 2013-10-30 Created: 2013-10-30 Last updated: 2015-05-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Ejeskär, Katarina
By organisation
School of Life SciencesThe Systems Biology Research Centre
In the same journal
Medical Oncology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 994 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf