This research focuses on a study of the material parameters of horse hoof. The study of the problem with the factors that affect to the fastening of the shoe is not performed. Three different tests are carried out to obtain the behavior of the horn wall of the horse hoof in different ways, under physiological conditions and variation of hydration level. The first one is a tensile test to obtain both the force/displacement relation and the stress/strain relation and the parameters derived from them. The second is a hardness test to determine how the material resists to several kinds of permanent-shape changes when a force is applied.Finally, a microscopic study is performed to analyze the fracture surface after testing the specimens. A meticulous analysis of the results and a broad comparison with several researches are performed. The end of the thesis work suggests future works needed to solve the problem.