his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Improved drought tolerance without undesired side effects in transgenic plants producing trehalose
University of Skövde, School of Life Sciences.
Department of Plant and Environmental Sciences, University of Göteborg, Box 461, Göteborg 405 30, Sweden.
University of Skövde, School of Life Sciences.
Department of Applied Biology, University of Helsinki, PL 27, Helsinki 00014, Finland.
Show others and affiliations
2007 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 64, no 4, 371-386 p.Article in journal (Refereed) Published
Abstract [en]

Most organisms naturally accumulating trehalose upon stress produce the sugar in a two-step process by the action of the enzymes trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Transgenic plants overexpressing TPS have shown enhanced drought tolerance in spite of minute accumulation of trehalose, amounts believed to be too small to provide a protective function. However, overproduction of TPS in plants has also been found combined with pleiotropic growth aberrations. This paper describes three successful strategies to circumvent such growth defects without loosing the improved stress tolerance. First, we introduced into tobacco a double construct carrying the genes TPS1 and TPS2 (encoding TPP) from Saccharomyces cerevisiae. Both genes are regulated by an Arabidopsis RuBisCO promoter from gene AtRbcS1A giving constitutive production of both enzymes. The second strategy involved stress-induced expression by fusing the coding region of ScTPS1 downstream of the drought-inducible Arabidopsis AtRAB18 promoter. In transgenic tobacco plants harbouring genetic constructs with either ScTPS1 alone, or with ScTPS1 and ScTPS2 combined, trehalose biosynthesis was turned on only when the plants experienced stress. The third strategy involved the use of AtRbcS]A promoter together with a transit peptide in front of the coding sequence of ScTPS1, which directed the enzyme to the chloroplasts. This paper confirms that the enhanced drought tolerance depends on unknown ameliorated water retention as the initial water status is the same in control and transgenic plants and demonstrates the influence of expression of heterologous trehalose biosynthesis genes on Arabidopsis root development.

Place, publisher, year, edition, pages
Springer Netherlands, 2007. Vol. 64, no 4, 371-386 p.
Keyword [en]
abiotic stress, arabidopsis, improved stress tolerance, tobacco, trehalose, trehalose-6-phosphate
National Category
Natural Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-6893DOI: 10.1007/s11103-007-9159-6ISI: 000247347200003PubMedID: 17453154Scopus ID: 2-s2.0-34249786589OAI: oai:DiVA.org:his-6893DiVA: diva2:575200
Available from: 2012-12-07 Created: 2012-12-07 Last updated: 2013-04-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Karim, SazzadEricson, HenrikHolmström, Kjell-Ove
By organisation
School of Life Sciences
In the same journal
Plant Molecular Biology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 1480 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf