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Molecular signature of cardiomyocyte clusters derived from human embryonic stem cells
University of Skövde, School of Life Sciences.ORCID iD: 0000-0003-4697-0590
Cellartis AB, Gothenburg, Sweden.
Cellartis AB, Gothenburg, Sweden.
Cellartis AB, Gothenburg, Sweden.
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2008 (English)In: Stem Cells, ISSN 1066-5099, Vol. 26, no 7, 1831-1840 p.Article in journal (Refereed) Published
Abstract [en]

Human embryonic stem cells (hESCs) can differentiate in vitro into spontaneously contracting cardiomyocytes (CMs). These cells may prove extremely useful for various applications in basic research, drug discovery, and regenerative medicine. To fully use the potential of the cells, they need to be extensively characterized, and the regulatory mechanisms that control hESC differentiation toward the cardiac lineage need to be better defined. In this study, we used microarrays to analyze, for the first time, the global gene expression profile of isolated hESC-derived CM clusters. By comparing the clusters with undifferentiated hESCs and using stringent selection criteria, we identified 530 upregulated and 40 downregulated genes in the contracting clusters. To further characterize the family of upregulated genes in the hESC-derived CM clusters, the genes were classified according to their Gene Ontology annotation. The results indicate that the hESC-derived CM clusters display high similarities, on a molecular level, to human heart tissue. Moreover, using the family of upregulated genes, we created protein interaction maps that revealed topological characteristics. We also searched for cellular pathways among the upregulated genes in the hESC-derived CM clusters and identified eight significantly upregulated pathways. Real-time quantitative polymerase chain reaction and immunohistochemical analysis confirmed the expression of a subset of the genes identified by the microarrays. Taken together, the results presented here provide a molecular signature of hESC-derived CM clusters and further our understanding of the biological processes that are active in these cells.

Place, publisher, year, edition, pages
2008. Vol. 26, no 7, 1831-1840 p.
Keyword [en]
human embryonic stem cells, differentiation, cardiomyocytes, gene expression
Identifiers
URN: urn:nbn:se:his:diva-6872DOI: 10.1634/stemcells.2007-1033ISI: 000258004400018PubMedID: 18436862Scopus ID: 2-s2.0-52949109296OAI: oai:DiVA.org:his-6872DiVA: diva2:573302
Available from: 2012-11-30 Created: 2012-11-30 Last updated: 2014-11-27Bibliographically approved

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CiteExportLink to record
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Citation style
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