his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The mitochondrial transporter SLC25A43 is frequently deleted and may influence cell proliferation in HER2-positive breast tumors
Clinical Research Centre, Örebro University Hospital, Örebro, Sweden / School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
University of Skövde, School of Life Sciences. University of Skövde, The Systems Biology Research Centre.
Show others and affiliations
2012 (English)In: BMC Cancer, ISSN 1471-2407, Vol. 12, Article number 350- p.Article in journal (Refereed) Published
Abstract [en]

Background: Overexpression of the human epidermal growth factor receptor (HER) 2 is associated with poor prognosis and shortened survival in breast cancer patients. HER2 is a potent activator of several signaling pathways that support cell survival, proliferation and metabolism. In HER2- positive breast cancer there are most likely unexplored proteins that act directly or indirectly downstream of well established pathways and take part in tumor development and treatment response.

Methods: In order to identify novel copy number variations (CNVs) in HER2-positive breast cancer whole-genome single nucleotide polymorphism (SNP) arrays were used. A PCR-based loss of heterozygosis (LOH) assay was conducted to verify presence of deletion in HER2-positive breast cancer cases but also in HER2 negative breast cancers, cervical cancers and lung cancers. Screening for mutations was performed using single-strand conformation polymorphism (SSCP) followed by PCR sequencing. Protein expression was evaluated with immunohistochemistry (IHC).

Results: A common deletion at chromosome Xq24 was found in 80% of the cases. This locus harbors the gene solute carrier (SLC) family 25A member 43 (SLC25A43) encoding for a mitochondrial transport protein. The LOH assay revealed presence of SLC25A43 deletion in HER2-positive (48%), HER2-negative (9%), cervical (42%) and lung (67%) cancers. HER2- positive tumors with negative or low SLC25A43 protein expression had significantly lower S-phase fraction compared to tumors with medium or high expression (P = 0.024).

Conclusions: We have found deletion in the SLC25A43 gene to be a common event in HER2-positive breast cancer as well as in other cancers. In addition, the SLC25A43 protein expression was shown to be related to S-phase fraction in HER2-positive breast cancer. Our results indicate a possible role of SLC25A43 in HER2-positive breast cancer and support the hypothesis of altered mitochondrial function in cancer.

Place, publisher, year, edition, pages
BioMed Central, 2012. Vol. 12, Article number 350- p.
Keyword [en]
Breast cancer, HER2, SLC25A43, S-phase
National Category
Biological Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-6540DOI: 10.1186/1471-2407-12-350ISI: 000309407100001PubMedID: 22883974Scopus ID: 2-s2.0-84864803696OAI: oai:DiVA.org:his-6540DiVA: diva2:560985
Available from: 2012-10-16 Created: 2012-10-16 Last updated: 2015-03-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Lubovac, Zelmina
By organisation
School of Life SciencesThe Systems Biology Research Centre
In the same journal
BMC Cancer
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 781 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf