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Using primary neuron cultures of Drosophila to analyze neuronal circuit formation and function
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, United Kingdom.
University of Skövde, School of Life Sciences. University of Skövde, The Systems Biology Research Centre. Cellartis, Göteborg, Sweden.
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, United Kingdom.
2012 (English)In: The making and un-making of neuronal circuits in Drosophila / [ed] Bassem A. Hassan, Springer Science+Business Media B.V., 2012, 225-247 p.Chapter in book (Refereed)
Abstract [en]

For many decades, primary neuron cultures of Drosophila have been used complementary to work in vivo. Primary cultures were instrumental for the analysis of physiological properties of Drosophila neurons and synapses, and they were used for the analysis of developmental processes. Recent developments have established Drosophila primary neurons based on Schneider's culture media, as a means to investigate the neuronal cytoskeleton, opening up novel opportunities for research into cellular mechanisms of axonal growth, synapse formation and perhaps even neuronal degeneration. These cell cultures provide readouts for cytoskeletal dynamics that are difficult or impossible to access in vivo, and which turned out to be highly conserved with mammalian or other vertebrate neurons. Therefore, the same genetic manipulations in Drosophila can now be studied synergetically in culture and in vivo, to address cell biological principles of neuronal circuit formation and function. Here we describe in detail how these cell cultures are generated and discuss principal considerations for the experimental design and the solution of common problems. Furthermore, we describe in detail how to generate Schneider's media with adjustable inorganic ion concentrations. These media have been shown to promote the physiological maturation of neurons, thus expanding the use of the primary neuron cultures into the synaptic stage. The culture strategies described here recapitulate in vivo development with impressive accuracy and provide a promising means for Drosophila research on neuronal development and function.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. 225-247 p.
Series
Neuromethods, ISSN 0893-2336 ; 69
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-5818DOI: 10.1007/978-1-61779-830-6_10Scopus ID: 2-s2.0-84867547076ISBN: 1-61779-829-0 ISBN: 978-1-61779-830-6 OAI: oai:DiVA.org:his-5818DiVA: diva2:524521
Available from: 2012-05-02 Created: 2012-05-02 Last updated: 2015-01-16Bibliographically approved

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Publisher's full textScopushttp://link.springer.com/protocol/10.1007/978-1-61779-830-6_10#

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CiteExportLink to record
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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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More styles
Language
  • de-DE
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