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Validation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues
University of Skövde, The Systems Biology Research Centre. University of Skövde, School of Life Sciences.
Örebro University Hospital.
Örebro University Hospital.
University of Skövde, The Systems Biology Research Centre. University of Skövde, School of Life Sciences.
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2010 (English)In: Cancer Genetics and Cytogenetics, ISSN 0165-4608, E-ISSN 1873-4456, Vol. 202, no 2, 71-75 p.Article in journal (Refereed) Published
Abstract [en]

When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used: small nuclear RNAs and small nucleolar RNAs. In this study, six different endogenous control genes for miRNA studies were investigated in prostate tissue material from the Swedish Watchful Waiting cohort. The stability of the controls was investigated using two different software applications, NormFinder and BestKeeper. RNU24 was the most suitable endogenous control gene for miRNA studies in prostate tissue materials.

Place, publisher, year, edition, pages
Elsevier Inc. , 2010. Vol. 202, no 2, 71-75 p.
National Category
Natural Sciences
Research subject
Natural sciences
Identifiers
URN: urn:nbn:se:his:diva-4712DOI: 10.1016/j.cancergencyto.2010.02.009ISI: 000282862400001PubMedID: 20875868Scopus ID: 2-s2.0-77957037880OAI: oai:DiVA.org:his-4712DiVA: diva2:394221
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Available from: 2011-02-02 Created: 2011-02-02 Last updated: 2014-02-25Bibliographically approved

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Carlsson, JessicaLubovac, ZelminaOlsson, BjörnKlinga-Levan, Karin
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
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