his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
1,25(OH)2D3 and Prostate Cancer: The Effects on cAMP/PKA-dependent Gene Expression in LnCaP cells
University of Skövde, School of Life Sciences.
2010 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Prostate cancer is the leading male cancer form i Sweden and maybe worldwide as well. Vitamin D is synthesized in the skin following the exposure to sunlight. Researcers have long been aware of the positive effect that vitamin D3 has on prostate tumour growth. 1,25(OH)2D3 have for a long time been the target of these studies and have shown good results. The steroid hormone induces cAMP accumulation and activiates the cAMP dependent protein kinaseA (PKA). PKA is then able to activate a transcription regulating protein. 1,25(OH)2D3 is known to cause LNCaP cells to accumulate in the G1 phase ofthe cell cycle. It has also been shown that 1,25(OH)2D3 is under negativefeedback control via 24-hydroxylase. In this study, PKA activity was observed by transfecting LNCaP cells with a viral vector carrying firefly and Renillaluciferase genes. The successfully transfected LNCaP cells would then express luciferase as a response to PKA gene expression. The LNCaP cells were then treated with 1,25(OH)2D3 and GDP-β-S (100μM), a G-protein coupled receptorinhibitor, in order to examine if 1,25(OH)2D3 regulate PKA dependent gene expression through a G-protein coupled receptor. The study could show that 1,25(OH)2D3 regulate gene expression in LNCaP cells through a PKAdependent pathway. Furthermore, the PKA dependent gene expression was demonstrated to be independent of G-protein coupled recpetor activation.

Place, publisher, year, edition, pages
2010. , 8 p.
Keyword [en]
prostate cancer, vitamin D, Vitamin D3, cAMP, PKA
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:his:diva-4466OAI: oai:DiVA.org:his-4466DiVA: diva2:372738
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2011-01-19 Created: 2010-11-26 Last updated: 2011-01-19Bibliographically approved

Open Access in DiVA

fulltext(210 kB)324 downloads
File information
File name FULLTEXT01.pdfFile size 210 kBChecksum SHA-512
a01ef5caa12aa27f3409f226be37e4ae2d1bce7c26cb17758ace7872dfde27d7ccfec03e94bbdac8554ada5626c52436fcd29ee6b4e86229cdeb066b5089fcbe
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Bergsten, Niklas
By organisation
School of Life Sciences
Pharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
Total: 324 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 309 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf