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Localization of AtHOG1 and AtHOG2 in Arabidopsis plants at the tissue and subcellular levels
University of Skövde, School of Life Sciences.
2010 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Plant hormones are responsible for plant growth and adaptation to the environment. Among them the most important are cytokinins. Plants undergo gene silencing processes called homology-dependent gene silencing processes. In Arabidopsis there are two homology-dependent gene silencing genes that were chosen for further study, namely AtHOG1 and AtHOG2. Transgenic plants were generated previously with ten different constructs containing AtHOG1 or AtHOG2 genes and were used in this study. Some of the constructs had GFP attached so that the protein expressed could be visualised in a confocal microscope. Transgenic plants generated were T1 and T2 generations. Their DNA was extracted from leaves. By means of PCR transgenic plants were identified. There were 147 samples. Among them there were 39 positiveswith BAR primers and 32 positives with construct specific primers. The localisation of the HOG2 protein was observed in a confocal microscope. Seeds used were T3 generation and were obtained from the lab. HOG2 protein was found to be localised in cell membrane, root tip and chloroplasts.

Place, publisher, year, edition, pages
2010. , 25 p.
Keyword [en]
transgenic Arabidopsis, transgenic plants, HOG1 gene
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:his:diva-4446OAI: oai:DiVA.org:his-4446DiVA: diva2:359859
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2010-11-04 Created: 2010-11-01 Last updated: 2010-11-04Bibliographically approved

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Biochemistry and Molecular Biology

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf