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Unravelling genetic mysteries: Next-generation sequencing in identifying disease-causing genes: A bioinformatic analysis
University of Skövde, School of Health Sciences.
2024 (English)Independent thesis Basic level (degree of Bachelor), 15 credits / 22,5 HE creditsStudent thesis
Abstract [en]

To identify disease-causing genes, numerous different ways are currently utilised. With the use of nextgeneration sequencing technology, different lengths of DNA, RNA, or entire genome sequences can be categorised simultaneously in a comparatively short amount of time. As a result, it is possible to generate a large amount of data in a single run, making this approach faster and more cost-effective than previously used methods. One frequently used next-generation sequencing method is wholeexome sequencing, which examines the complete coding sequence of every exon in an organism. The exon makes up just 1% of the whole genome, but it includes coding regions with a significant portion of small insertions and deletions as well as single nucleotide variations that are now known to cause Mendelian diseases. This study aimed to investigate possible disease-causing genes for a given rare genetic disorder using platforms QIAGEN - Clinical Insight and Ensemble. Exome data from a patient with a rare genetic condition together with clinical information and a family pedigree were provided. Data from whole exome sequencing was provided in the form of a VCF file uploaded to the QIAGEN - Clinical Insight platform for bioinformatic analysis. Through the process of elimination, the genes were narrowed down to 8 candidate genes that could present a valuable option for further research on the possible cause of the disease.  

Place, publisher, year, edition, pages
2024. , p. 1, 30
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:his:diva-24038OAI: oai:DiVA.org:his-24038DiVA, id: diva2:1875542
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Study Programme
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Available from: 2024-06-23 Created: 2024-06-23 Last updated: 2024-06-23Bibliographically approved

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