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Testosterone induced PCOS-like traits in drosophila melanogaster
University of Skövde, School of Health Sciences.
2024 (English)Independent thesis Basic level (degree of Bachelor), 15 credits / 22,5 HE creditsStudent thesis
Abstract [en]

Polycystic ovary syndrome is a disorder affecting 15-20% of women of reproductive age and are the leading cause of infertility worldwide. According to the Rotterdam diagnostic criteria two out of three symptoms need to be present: oligo and/or anovulation, clinical or biochemical hyperandrogenism and polycystic ovarian syndrome. In 80% hyperandrogenism are present and is thus considered a hallmark of this disorder. Drosophila melanogaster is a useful model for disease and treatment studies due to their short generation cycle. The aim of this project was to establish a PCOS-like model in drosophila,induced by two different concentrations (0.1mg/μl and 0.01mg/μl) of testosterone to generate phenotypical changes associated with the disorder. By breeding two consecutive generations of female flies, the first generation with treatment and the second without and assess fecundity, measure to tallipid levels and gene expression of PCOS related pathways (genes: THADA, FOXO and Spo) with qPCR between the different treatment groups and between the two generations. Results showed a trend towards a mild metabolic phenotype in the high testosterone-exposed flies via increased expression of FOXO and THADA and higher lipid levels for both treatment groups 1.78 and 1.74 compared to control 1.53 in the first generation. For the second generation only, fecundity was assessed and showed no statistical difference from the first to the second (p=0.414) or between treatment groups which means no reproductive disturbances. An optimised model could have merit for future research into PCOS, if not as a whole model, then at least into some aspects.

Place, publisher, year, edition, pages
2024. , p. 17
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:his:diva-23993OAI: oai:DiVA.org:his-23993DiVA, id: diva2:1873451
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Study Programme
Supervisors
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Available from: 2024-06-19 Created: 2024-06-19 Last updated: 2024-06-19Bibliographically approved

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CiteExportLink to record
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Citation style
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