Type 1 Diabetes Mellitus (T1D) is a chronic autoimmune disorder, characterized by the destruction of insulin-producing pancreatic Beta cells, mediated by the immune system T- cells. Worldwide, 1,110,100 individuals are affected by T1D. The exact mechanism by which the disease manifest itself is still unclear and is likely to be the result of a complex interplay between genetic predispositions, diet, environmental triggers. The gut microbiome has gained a growing interest in T1D research. The microbiome composition is greatly influenced by diet. Dysbiosis could lead to disease through various mechanism. One such mechanism is through the influence of small molecules such as bacterial metabolites, on cellular processes. The aim of this study is to investigate the role of Gamma aminobutyric acid GABA as one of those bacterial metabolites’ influences on the Forkhead box o1 gene, involved in beta cell proliferation and differentiation. Through a four-week dietary intervention aimed to increase bacterial GABA. Using methods like qPCR, amino acids profiling, and external blood panel analysis. The results showed a significant increase in FOXO1 With an average relative expression of 6.41 before, compared to 14.48 after the diet. p=0.026, α= 0.05. There was a significant quadratic correlation between change in taurine and change in FOXO1 R2=0.96, p=0.045. And a non-significant linear correlation R2=0.82, p=0.368, between changes in GABA and FOXO1. The results suggested that diet may influence certain genes relative to T1D, however it is difficult to establish cause and effect in complex physiological systems.