Type 1 diabetes is an autoimmune disease characterized by its insulin dependence. This autoimmune disease is characterized in the destruction of pancreatic B cells through the instigation of the T-cell and humoral responses. Type 1 diabetes and celiac disease share predisposing genetic association in its rise of autoimmunity approach where the exclusions of gluten alleviate arising symptoms. The diabetic incidence was analyzed from the blood glucose measurement data of the NOD mice to determine which diets affect the diabetic incidence. Blood glucose measurement above 12 mmol/l was determined diabetic. A statistical method Kaplan-Meier was used to analyze the diabetic incidence in response to the exposure of different diets. Two-way ANOVA and Bonferroni correction was conducted on body weight of the NOD mice. Quantitative PCR using the TaqMan method was used to analyze the gene expression level, independent sample test was used to compare the relative expression. The different gene expression was analyzed in different tissues of the gastrointestinal tract such as colon, duodenum and ileum. We found that Pck1, Fbp1, Gstm1, Slc2a2 and Ifi44 were differentially expressed in response to gluten free diet compared to standard. The genes studied encodes regulatory enzymes for gluconeogenesis, inflammation mediators, and the facilitation of glucose transport. A gluten free diet provided a reduction in the diabetic incidence in comparison to the other diets administered. A gluten free diet might be a favorable therapeutic approach in reducing, delaying or even prevent the onset of type 1 diabetes.