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The impact of p13α expression in the adult fruit fly exposed to high sugar diet elucidating the molecular link between diabetes and cancer
University of Skövde, School of Health Sciences.
2021 (English)Independent thesis Basic level (degree of Bachelor), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Diabetes is one of the most common metabolic disorders in the world. Studies have shown that people with diabetes have a higher risk of developing cancer. P13α is a splice variant of PIK3CA gene which is often mutated in cancer linked to diabetes. Glucose metabolism and lipogenesis is dysregulated in type 2 diabetes. Gluconeogenesis, non-oxidative glycolysis and lipogenesis are increased, while the pentose phosphate pathway (PPP) is reduced. In this study the effect of p13α expression on Drosophila Melanogaster fed on high sugar diet (HSD) compared to normal diet was analyzed. Developmental speed, length and weight measurements were taken. Rate of survival and relative gene expression of six genes involved in glucose metabolism and lipogenesis were analyzed and compared within and between diets. Results obtained showed that p13α flies developed faster compared to controls on both diets. P13α male flies had lower survival rate on both diets. P13α flies on normal diet had higher average weight than control and lower average weight on HSD compared to control. P13α relative expression was reduced in the HSD compared to normal diet. Results obtained from gene expression analysis indicated differences in expression of all six genes analyzed in p13α flies compared to control flies; however the differences were not significant. In conclusion p13α expression in Drosophila seems to have an effect on development, weight and survival of the flies. However further research is needed in order to make a concrete conclusion on its effect on gene expression of the genes that were analysed.

Place, publisher, year, edition, pages
2021. , p. 35
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:his:diva-20552OAI: oai:DiVA.org:his-20552DiVA, id: diva2:1593304
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Study Programme
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Available from: 2021-09-11 Created: 2021-09-11 Last updated: 2021-09-11Bibliographically approved

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