Diabetes and cancer are the most frequent types of disease that result in severe health complications.Evidence suggests that individuals with diabetes type 2 are in higher risk factor for developing certaincancer types, such as breast cancer and endometrial cancer. PIK3CA is a protein involved in cancer.p13𝛼 is PIK3CA isoform that encodes for a smaller protein which only contain one intact domain, thep85 binding domain. The insulin signaling pathway in the fruit fly, Drosophila Melanogaster, resemblesto that of humans. The aim of the study was to find out if there was any effect on the size andmetabolism of the fruit fly larvae if p13𝛼 was over expressed., also to examine whether larvaeoverexpressing p13𝛼 were more or less sensitive to a diet high in sugar. The metabolism wasinvestigated by analysis of gene expression of lipogenes and gluconeogenesis genes. Results indicatedthat there was a significant difference between the weight (g) and size (mm) of the larvae in normaldiet as compared to high sugar diet. Gene expression analysis implied that there was a differencebetween the larvae received the high sugar diet as compared to normal diet. In conclusion, the weight(g), size (mm) and metabolism of the larvae were proven to be effected in response to overexpressionof p13𝛼.