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Longitudinal study of memory B cells postpartum
University of Skövde, School of Bioscience.
2021 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The adaptive arm of the immune system protects from reinfection and is recognized by an immense diversity of cells that interact and coevolve as novel pathogens emerge. At gestation, the mother undergoes profound and complex changes of her immune system to maintain tolerance towards the fetus while providing proper protection against any intruders. Understanding the mechanisms behind this balancing act can help identify the hallmarks of pregnancy well-being and adverse outcomes. Here, the aim was to explore the dynamic changes at gestation and after delivery in sorted B cell populations including naïve and two memory B cell (MBC) subsets named herein CD27dull and CD27bright from three pregnant females. The subsets were analyzed using Illumina MiSeq HTS data derived from the full-length immunoglobulin heavy chain variable region transcript incorporated with unique molecular identifiers (UMIs). The data was processed using UMI-guided error corrections and reads with shared sequence identities were assembled into clonotypes. The samples were further analyzed by comparing baseline B cell repertoire properties including variable gene segment usage (IGHV), complementarity determining region 3 (CDR3) sizes and levels of somatic hypermutations (SHMs). The results revealed a dynamic change in IGHV usage profiles and an increase of CDR3 sizes between gestation and after delivery. CD27bright exhibited higher levels of SHMs than CD27dull, and SHMs tend to accumulate unequally across the region. CD27bright increased their replacement-to-silent SHM ratio after delivery. The results shed more light on maternal immune adaptation and provide a framework that can be refined and optimized in future works.

Place, publisher, year, edition, pages
2021. , p. 51
National Category
Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:his:diva-19928OAI: oai:DiVA.org:his-19928DiVA, id: diva2:1569727
External cooperation
University of Gothenurg
Subject / course
Systems Biology
Educational program
Molecular Biotechnology - Master's Programme, 120 ECTS
Supervisors
Examiners
Available from: 2021-06-21 Created: 2021-06-21 Last updated: 2021-06-21Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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More styles
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  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
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