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FGFlncRNA1 mediated chemokine activity in cancer
University of Skövde, School of Health Sciences.
2021 (English)Independent thesis Basic level (degree of Bachelor), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Long noncoding RNAs are a highly heterogenous class of compounds and versatile regulators of the human genome. While several are identified and annotated, only a few have been functionally characterised. The Kanduri lab has established that FGFlncRNA1 is upregulated in several cancers and is involved in tumour cell proliferation and invasion. The aim of this study was to analyse the FGFlncRNA1/chemokine functional nexus in cancer. CCL20 and CXCL1 were identified as downstream targets of the lncRNA. Knockdown of the chemokines resulted in a significant reduction of proliferation, migration, and invasion. Furthermore, western blot analysis revealed the involvement of two pathways, MAPK/ERK and PI3K/AKT. These were detected through phosphorylated ERK and phosphorylated AKT protein levels. Overall, the present study demonstrated that FGFlncRNA1 may function as an oncogene in cancer progression by modulating CXCL1 and CCL20 activity, which assert their downstream activity through ERK and AKT signalling. This study bridges a small gap in the scientific community’s quest to decode the role of lncRNAs in cancer.

Place, publisher, year, edition, pages
2021. , p. 29
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:his:diva-19881OAI: oai:DiVA.org:his-19881DiVA, id: diva2:1568701
External cooperation
The Kanduri Lab (Göteborgs universitet)
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Study Programme
Supervisors
Examiners
Available from: 2021-06-17 Created: 2021-06-17 Last updated: 2021-06-17Bibliographically approved

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