Obesity is a worldwide epidemic that can cause various health issues of different magnitudes. Recent findings have shown oxytocin’s involvement in regulating feeding behavior. Oxytocin communicates with the brain through circulation or nerves to signal satiety, consequently suppressing food intake. This suggests a potential treatment for obesity using oxytocin. Gastrointestinal tract was hypothesized to be a peripheral source of oxytocin system, thus expression of Oxt and Oxtr was investigated using 6GIT tissues of 12 male and 12 female rats, among which 6 were fasted for 16 hours and 6 were re-fed for 45 minutes. Then qPCR was performed to detect expressions. Oxt was not detected in any tissues while Oxtr was detected in the cecum and distal stomach in the original experiment and all tissues in the validation. In the original experiment, no significant differences in Oxtr expression between feeding conditions in male (p=1.000) and female (p=0.589) and between sexes in fasted (p=0.818) and re-fed (p=0.937) conditions were found in cecum. The validation showed no significant difference in Oxtr expression between sexes (p=0.293). Lastly, no significant differences in Oxtr expression between sexes in cecum (p=0.385; 95% CI= -5.589/2.685) and distal stomach (p=0.368; 95% CI= -3.451/1.605) were found. In conclusion, Oxt was not detected in the 6 GIT tissues while Oxtr was detected, however no significant differences were found to support food intake modulates Oxt and Oxtr expressions. Further research is needed to understand the mechanisms of anorexigenic effect of oxytocin to be utilized as a potential treatment for obesity.