Diabetes Mellitus Type I (DMT1) is a disease characterised by the autoimmune destruction of β-cells in the pancreas. As a result, glucose homeostasis is altered, and severe hyperglycaemia and hypoglycaemia episodes can occur, risking the patient's health and life. DMT1 prevalence has increased in the past years, being linked to a rise in obesity and Body Mass Index. Although treated patients have a relatively good quality of life, new treatment approaches and preventive strategies are being investigated. Previous studies have reported the protective impact a gluten-free diet has for DMT1 pathogenesis, but how it effectuates its effect is yet unknown. In this experiment, gene expression was studied in different parts of the gastrointestinal tract of nonobese diabetic mice (NOD mice) who received either a gluten-free or a gluten-containing diet. Genes studied codified for gluconeogenesis enzymes, glucose and amino acids transporters, gene expression regulatory enzymes and inflammation mediators. The methods used were RT-qPCR for gene expression quantification and the 2-ΔΔCT Method to analyse the data obtained. A One-Sample T-test and a Bonferroni correction were done, and Pck1, Fbp1, Gstm3 and Ifit1 were found to be differentially expressed between the gluten-free and control groups. A correlation between gluten intake and inflammation seems to exist, although further gene expression analysis should be performed in the liver to determine precisely how gluconeogenesis varies in both organs. If results were to be favourable, a gluten-free diet could be advised for individuals genetically predisposed to DMT1 to prevent the disease.