High-throughput genotyping which results in identification and mapping a vast number of single nucleotide polymorphisms help improving the performance of genome-wide association studies and moreover, connecting DNA variants to phenotypes of target. Many genotyping protocols are depended on polymerase chain reaction (PCR) amplification of genes or markers, since PCR is an easy, fast, sensitive, and cost effective technique. The main aim of this study was to set up a method for identifying the important genotypes of stress and sense of coherence. Since recent studies on human have shown that, oxytocin secretion decrease in response to stress, this study investigated those likely associations among single-nucleotide polymorphisms (SNPs) in the rs53576 and rs225498 of oxytocin receptor (OXTR) gene with stress. Moreover, SNPs in the 5-HTTLPR gene were examined as polymorphisms in 5' region of serotonin-transporter (5-HT) gene (17q11.2, 5-HTTLPR) causing changes in the transcription of 5-HT gene, which are apparently correlated with personality traits such as, depression, anxiety, and stress in human. In this experiment the extracted DNA from the blood samples were used to analyze two polymorphisms by using PCR and Fragment Analyzer techniques. To set up the PCR experiment different reagents such as dNTPs amount and also the annealing temperature were evaluated to get the optimal results. Based on the obtained results from the fragment analysis and data analysis the set up method was work meanwhile hypotheses was rejected. There were no significant differences between these two polymorphism sites of OXTR and 5HTTLPT from serotonin receptor gene with stress and sense of coherence (SOC). Therefore the limitation of samples selection can be considered as one of the major reasons for rejecting the hypothesis, so in next experiments the more number of participants with more specific category (for example, age, sex, different region) can be used.