Type 1 Diabetes (T1D) is an autoimmune disease that results in destruction of the pancreatic β cells. T1D is related to another autoimmune disease - Celiac disease(CD). Studies have shown that patients diagnosed with both T1D and CD, generally develop T1D first, whereas patients that have been diagnosed with CD display a lower risk of developing T1D. As patients diagnosed with CD exclude gluten from their diets, this lead to a hypothesis that gluten dietary intake may play a role in T1D onset. This study was aimed to investigate the anti-diabetic effects of the gluten-free diet on a cohort of non-obese diabetic (NOD) mice and determine whether genes associated with diabetes are higher or lower expressed in NOD mice when consuming a gluten-free diet compared to a standard chow diet. qPCR combined with the ΔΔCt method has been used to analyze gene expression of 20 genes of interest compared to four reference genes in the two groups of NOD mice. Ten genes: Slc6a19, Spint1, Gstm3, Gls, Fbp1, Fbp2, Slc2a2, Ifit1, St14 and Hgfac showed a significant difference in gene expression between the two diet groups. Thus, a correlation between dietary gluten intake and expression of these diabetes-related genes is suggested. Future research will be aimed at understanding the pathways triggering the onset of T1D in the genetically predisposed individuals. This could lead to possible prevention therapy in form of a gluten-free diet, which may lead to lowered incidence of T1D in the world.