Inflammasomes are large multiprotein complexes that are part of the innate immune response and assemble in response to microbial microorganisms. The function of the innate immune response is dependent upon the detection of pathogen-associated molecular patterns and Damage associated-molecular patterns by germline-encoded pattern recognition receptors. The NLRP3 is the most characterized member of the NOD-like receptors that are also capable of inflammasome activation, the activation leads to the cleavage of caspase-1 into its active form. Dysregulation of inflammasome activation has been linked to several autoimmune diseases such as type1 and 2 diabetes, and the mechanisms regulating the activation of the NLRP3 activation are not fully understood yet. This study aims to study the time dependence of processes related to the activated NLRP3 inflammasome. THP-1 cells were cultured and collected at set time points and the first objective was to perform a western blot analysis and the second was to do an RT-qPCR. The obtained results from the qPCR indicated that they were multiple products in the samples and therefore unspecific amplification during the run. Troubleshooting tests such as melting curve analysis was used to determine the melt profile of the amplicon and gel electrophoresis was used to determine if they were multiple products in the samples. The results from the western blot analysis showed unexpected bands and due to time constraints, further optimization for the antibodies could not be performed.