his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood-brain barrier model
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Department of Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Sweden / Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Mölndal, Sweden. (Translationell Bioinformatik, Translational Bioinformatics)ORCID iD: 0000-0003-2899-3801
BioLamina, Sundbyberg, Sweden.
Department of Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Sweden / Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden / Department of Neurodegenerative Disease, UCL Institute of Neurology, London, UK / UK Dementia Research Institute at UCL, London, UK.
Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Mölndal, Sweden.
Show others and affiliations
2019 (English)In: Fluids and Barriers of the CNS, ISSN 2045-8118, E-ISSN 2045-8118, Vol. 16, no 1, article id 27Article in journal (Refereed) Published
Abstract [en]

Background Human induced pluripotent stem cells (hiPSC) hold great promise for use in cell therapy applications and for improved in vitro models of human disease. So far, most hiPSC differentiation protocols to astroglia use undefined, animal-containing culture matrices. Laminins, which play an essential role in the regulation of cell behavior, offer a source of defined, animal-free culture matrix. Methods In order to understand how laminins affect astroglia differentiation, recombinant human laminin-521 (LN521), was compared to a murine Engelbreth-Holm-Swarm sarcoma derived laminin (L2020). Astroglia expression of protein and mRNA together with glutamate uptake and protein secretion function, were evaluated. Finally, these astroglia were evaluated in a coculture model of the blood-brain barrier (BBB). Results Astroglia of good quality were generated from hiPSC on both LN521 and L2020. However, astroglia differentiated on human LN521 showed higher expression of several astroglia specific mRNAs and proteins such as GFAP, S100B, Angiopoietin-1, and EAAT1, compared to astroglia differentiated on murine L2020. In addition, glutamate uptake and ability to induce expression of junction proteins in endothelial cells were affected by the culture matrix for differentiation. Conclusion Our results suggest that astroglia differentiated on LN521 display an improved phenotype and are suitable for coculture in a hiPSC-derived BBB model. This provides a starting point for a more defined and robust derivation of astroglia for use in BBB coculture models.

Place, publisher, year, edition, pages
BioMed Central, 2019. Vol. 16, no 1, article id 27
Keywords [en]
Astroglia, hiPSC, In vitro models, Diferentiation, Laminin-521, Blood–brain barrier
National Category
Pharmaceutical Sciences
Research subject
Bioinformatics
Identifiers
URN: urn:nbn:se:his:diva-17672DOI: 10.1186/s12987-019-0147-4ISI: 000483547700001PubMedID: 31462266Scopus ID: 2-s2.0-85071630761OAI: oai:DiVA.org:his-17672DiVA, id: diva2:1350718
Available from: 2019-09-12 Created: 2019-09-12 Last updated: 2019-11-13Bibliographically approved

Open Access in DiVA

fulltext(5070 kB)53 downloads
File information
File name FULLTEXT01.pdfFile size 5070 kBChecksum SHA-512
a7586d1cdcf7018654eb08d56c1fec758ed3f9097f07a35058542e02875c2a6934682ed6834f9887b1e7c4ca76b856cd87013c882d7119a89ad0f30f495298aa
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records BETA

Delsing, LouiseSynnergren, Jane

Search in DiVA

By author/editor
Delsing, LouiseSynnergren, Jane
By organisation
School of BioscienceThe Systems Biology Research Centre
In the same journal
Fluids and Barriers of the CNS
Pharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 53 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 205 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf