his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Functinal analysis of an arsenic reductaseregulatory arsC gene (Gene ID: 1254) isolatedfrom Lysinibacillus sphaericus B1-CDA
University of Skövde, School of Bioscience.
2018 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Arsenic is a metalloid compound and it has become a severe threat to human health and environment when exceeding their maximum permissible limit of 0.01 mg/L in drinkingwater. Arsenic is a toxic and carcinogen substance which acts as an inhibitor for oxidativephosphorylation in our body and as a result, many diseases like skin cancer, stomach pain,kidney failure, cardiovascular disease etc can be found in human. So removal of arsenic from contaminated area is of great importance for human welfare. The main objective of this study was to functional analysis of arsC gene found in Lysinibacillus sphaericus which was capable of removing arsenic from arsenic contaminated LB medium. The Iterative Threading Assembly Refinement (I‐TASSER) tools were used to predict the secondary structure,molecular and biological annotation and 3D structure of arsC protein. Based on these analyses the function of this gene was predicted. The arsC gene was isolated from L.sphaericus and transferred to an arsC‐deficient strain of Escherichia coli MG 1655. RT‐PCR, colony PCR and blue white screening methods were used to confirm the insertion of this gene into this strain. Both transgenic and non‐transgenic strains of E.coli were exposed to arsenic stress and their tolerance and growth were studied. The transgenic strain was found to be more tolerant and could grow better when exposed to arsenics. These results suggest that the arsC gene of Lysinibacillus sphaericus B1‐CDA can be used as a potential candidate for genetic engineering to efficiently remove arsenics from contaminated sources like soil,water or industry effluents.

Place, publisher, year, edition, pages
2018.
Keywords [en]
Arsenic, I-TASSER, RT‐ PCR, Colony PCR, Lysinibacillus sphaericus B1 ‐ CDA, Human health, Bioremediation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:his:diva-16424OAI: oai:DiVA.org:his-16424DiVA, id: diva2:1265341
Subject / course
Molecular Biology
Educational program
Molecular Biotechnology - Master’s Programme, 60 ECTS
Supervisors
Examiners
Available from: 2018-11-25 Created: 2018-11-23 Last updated: 2018-11-25Bibliographically approved

Open Access in DiVA

No full text in DiVA

Search in DiVA

By author/editor
Rahman, Mohammad Mahmudur
By organisation
School of Bioscience
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 65 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf