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In vivo assessment of focal adhesion kinase (FAK) activity in breast cancer cells using fluorescence resonance energy transfer (FRET) sensor and confocal laser scanning microscope (CLSM)
University of Skövde, School of Health and Education.
2015 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Focal Adhesion Kinase (FAK) is essential for cell migration and plays an important role in tumor metastasis. However, the complex intermolecular and intramolecular interactions that regulate FAK activity at the focal adhesion remain unresolved. We have engineered a toolbox of Fluorescence Resonance Energy Transfer (FRET) sensors for the assessment of FAK activity in human breast cancer cells (MCF-7). Major activity of cancerous cells is drastically growth of the cell in an uncontrollable manner in such cases our human anatomy system normally consists of cell growth activity. The important protein involved in cell functionality in the human body is FAK, due to FAK activity, cell motility, proliferation, survival has been managed in the human body hence, it is necessary to investigate the performance ofFAK activity on breast cancer becomes important.

In our study, the differences in bleed through between zoom = 1 and for zoom >1 for donor and acceptor was evaluated. There were no significant differences in Pearson correlation coefficient and bleed through coefficient for both the zooms. With recent advances influorescent probes, instrumentation and methodologies, FRET is sure to revolutionize scientific research in the near future.

Place, publisher, year, edition, pages
2015. , p. 29
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:his:diva-15706OAI: oai:DiVA.org:his-15706DiVA, id: diva2:1220943
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Master's Programme
Supervisors
Examiners
Available from: 2018-06-20 Created: 2018-06-19 Last updated: 2018-06-20Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf