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Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. (Bioteknik, Biotechnology)
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. (Bioteknik, Biotechnology)ORCID iD: 0000-0002-8162-8945
Microbial Diversity Research Centre, Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune, India.
School of Arts and Science, Iona College, New Rochelle, NY, USA.
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2017 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 218, 121-126 p.Article in journal (Refereed) Published
Abstract [en]

We have cloned, characterized and transformed the AtACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into the genome of tobacco (Nicotiana tabacum, var Sumsun). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild type ones. These plants can grow on culture medium containing 200μM arsenate, whereas the wild type can barely survive under this condition. Furthermore, when exposed to 100μM arsenate for 35days the amount of arsenic accumulated in the shoots of transgenic plants was significantly lower (28μg/g d wt.) than that found in the shoots of non-transgenic controls (40μg/g d wt.). However, the arsenic content in the roots of transgenic plants was significantly higher (2400μg/g d. wt.) than that (2100μg/g d. wt.) observed in roots of wild type plants. We have demonstrated that Arabidopsis thaliana AtACR2 gene is a potential candidate for genetic engineering of plants to develop new crop cultivars that can be grown on arsenic contaminated fields to reduce arsenic content of the soil and can become a source of food containing no arsenic or exhibiting substantially reduced amount of this metalloid.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 218, 121-126 p.
Keyword [en]
Arabidopsis thaliana, Arsenic, AtACR2 overexpression, Heavy metal accumulation, Nicotiana tabacum, Phytoremediation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:his:diva-14271DOI: 10.1016/j.jplph.2017.08.001ISI: 000413327800014PubMedID: 28818758Scopus ID: 2-s2.0-85031780264OAI: oai:DiVA.org:his-14271DiVA: diva2:1153525
Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2017-11-10Bibliographically approved

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