Högskolan i Skövde

The hydrolysis of the polycaprolactone (PCL) as a function of the dissolution time in a formic/acetic acid mixture was considered as a method for tailoring the morphology of nanofibrous PCL scaffolds. Hence the aim of this research was to establish a correlation between the dissolution time of the polymer in the acid solvent with the physicochemical properties of the electrospun nanofibrous scaffolds and their further service life behaviour. The physico-chemical properties of the scaffolds were assessed in terms of fibre morphology molar mass and thermal behaviour. A reduction of the molar mass and the lamellar thickness as well as an increase of the crystallinity degree were observed as a function of dissolution time. Bead-free fibres were found after 24 and 48 h of dissolution time with similar diameter distributions. The decrease of the fibre diameter distributions along with the apparition of beads was especially significant for scaffolds prepared after 72 h and 120 h of dissolution time in the acid mixture. The service life of the obtained devices was evaluated by means of in vitro validation under abiotic physiological conditions. All the scaffolds maintained the nanofibrous structure after 100 days of immersion in water and PBS. The molar mass was barely affected and the crystallinity degree and the lamellar thickness increased along immersion preventing scaffolds from degradation. Scaffolds prepared after 24 h and 48 h kept their fibre diameters whereas those prepared after 72 h and 120 h showed a significant reduction. This PCL tailoring procedure to obtain scaffolds that maintain the nanoscaled structure after such long in vitro evaluation will bring new opportunities in the design of long-term biomedical patches.