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Outcomes of Myosin 1C Gene Expression Depletion on Cancer-related Pathways, in Vitro and in Clinical Samples
Sahlgrenska Academy at University of Gothenburg. (Tumor Biology)
2016 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

The unconventional myosin IC has previously been suggested to be a haploinsufficient tumour suppressor. The mechanism for this action has hitherto been unknown, however, and hence we decided to attempt to elucidate the genes involved. The first study involved knock-down of MYO1C using siRNA technology followed by whole transcriptiome microarray analysis performed on samples taken at different time points post transfection. This revealed a cornucopia of differential expressions compared to the negative control, among them we found an early up-regulation of the PI3K/AKT pathway and the pathway for prostate cancer. Among the down regulated pathways we found endometrial-, colorectal cancer and small cell lung cancer as well as the cell cycle pathway which was a little counter intuitive to the hypothesis that MYO1C suppresses cancer. For the next study six different genes (CCND1, CCND2, CDKN2B, CDKN2C, MYC, RBL1) important for the transitions into S-phase of the cell cycle were therefore chosen for validation using qPCR. These six genes and MYO1C were analysed on both the original time series and a new biological replicate as well as a well stratified set of endometrial carcinoma samples. We were able to verify the significant down-regulation of CCND2 in both time series indicating that this is caused by the depletion of MYO1C. In the tumour samples we saw a negative correlation between the expression of MYO1C and FIGO grade corroborating results previously found by our group when looking at protein expression.

Place, publisher, year, edition, pages
University of Gothenburg, 2016. , p. 86
Keywords [en]
MYO1C, myosin 1C, gene expression, pathway analysis, endometrial carcinoma
National Category
Basic Medicine Medical Genetics
Identifiers
URN: urn:nbn:se:his:diva-12981ISBN: 978-91-639-1067-8 (print)OAI: oai:DiVA.org:his-12981DiVA, id: diva2:1034158
Presentation
2016-09-02, 13:00 (English)
Supervisors
Available from: 2017-11-21 Created: 2016-09-28 Last updated: 2018-01-14Bibliographically approved

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Pfister, Anna

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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  • asciidoc
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